Western Blot (WB)
(IKKa mAb tested by Western blot. IKKa detection by Western blot analysis. The detection of IKKa was performed using 30 ug of whole-cell extract from Daudi cells with the IKKa mAb at a 1 ug/ml dilution.)
Mouse anti-Human, Mouse IKKa Monoclonal Antibody | anti-IKKa antibody
IKKa antibody (mAb)(Clone 14A231)
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purified
Synonyms
IKKa; Monoclonal Antibody; IKKa antibody (mAb)(Clone 14A231); CHUK; IKK1; IKBKA; TCF16; NFKBIKA; IKK-alpha; anti-IKKa antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
14A231
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-IKKa antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This IKKa antibody was raised against a full-length protein corresponding to human IKKa.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(IKKa mAb tested by Western blot. IKKa detection by Western blot analysis. The detection of IKKa was performed using 30 ug of whole-cell extract from Daudi cells with the IKKa mAb at a 1 ug/ml dilution.)
Western Blot (WB)
(IKKa mAb tested by Western blot. IKKa detection by Western blot analysis. The detection of IKKa was performed using 30 ug of whole-cell extract from Daudi cells with the IKKa mAb at a 1 ug/ml dilution.)
Related Product Information for anti-IKKa antibody
Short Description: Our IKKa antibody (mAb)(Clone 14A231) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human and Mouse samples.
Background: IKKa (IkB Kinase alpha subunit)-The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB (NFkB p50 & NFkB p65). The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IkBa at Ser32 and Ser36. This results ultimately in the degradation of IkB and the activation and nuclear translocation of NFkB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK-beta appears to be the principal kinase, as IKKa is not required for activation of the IKK complex and the subsequent degradation of IkBa.
Background: IKKa (IkB Kinase alpha subunit)-The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB (NFkB p50 & NFkB p65). The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IkBa at Ser32 and Ser36. This results ultimately in the degradation of IkB and the activation and nuclear translocation of NFkB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK-beta appears to be the principal kinase, as IKKa is not required for activation of the IKK complex and the subsequent degradation of IkBa.
Product Categories/Family for anti-IKKa antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,640 Da
NCBI Official Full Name
inhibitor of nuclear factor kappa-B kinase subunit alpha
NCBI Official Synonym Full Names
conserved helix-loop-helix ubiquitous kinase
NCBI Official Symbol
CHUK
NCBI Official Synonym Symbols
IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
NCBI Protein Information
inhibitor of nuclear factor kappa-B kinase subunit alpha
NCBI Description
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]
Research Articles on IKKa
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Product Notes
The IKKa (Catalog #AAA388669) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IKKa antibody (mAb)(Clone 14A231) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IKKa can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the IKKa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKKa, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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