Western Blot (WB)
(CHD1 antibody (pAb) tested by Western blot. Detection of CHD1 by Western blot analysis. Lane 1: Nuclear extract of HeLa cells (50 ug). Lane 2: Whole cell extract of adult mouse brain. Both probed with CHD1 antibody (pAb) at a 1:1,000 dilution.)
Rabbit anti-Human, Mouse CHD1 Polyclonal Antibody | anti-CHD1 antibody
CHD1 antibody (pAb)
WB: 1:500-1:1,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Western Blot (WB)
(CHD1 antibody (pAb) tested by Western blot. Detection of CHD1 by Western blot analysis. Lane 1: Nuclear extract of HeLa cells (50 ug). Lane 2: Whole cell extract of adult mouse brain. Both probed with CHD1 antibody (pAb) at a 1:1,000 dilution.)
Western Blot (WB)
(CHD1 antibody (pAb) tested by Western blot. Detection of CHD1 by Western blot analysis. Lane 1: Nuclear extract of HeLa cells (50 ug). Lane 2: Whole cell extract of adult mouse brain. Both probed with CHD1 antibody (pAb) at a 1:1,000 dilution.)
Background: CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD1 has been shown to interact with histone H3 trimethylated at lysine 4 (H3K4Me3) and also is required for the maintenance of stem cell pluripotency.
NCBI and Uniprot Product Information
NCBI Description
The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. [provided by RefSeq, Jul 2008]
Uniprot Description
CHD-1: a sequence-selective DNA-binding protein. Contains two chromo (chromatin organization modifier) domains and one SNF2-related helicase and ATPase domains. May play an important role in gene regulation.
Protein type: Helicase; EC 3.6.4.12
Chromosomal Location of Human Ortholog: 5q15-q21
Cellular Component: cytoplasm; nucleus
Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; methylated histone residue binding; ATP binding
Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; DNA duplex unwinding
Research Articles on CHD1
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Product Notes
The CHD1 chd1 (Catalog #AAA388556) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHD1 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CHD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the CHD1 chd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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