Rabbit RbAp46/48 Polyclonal Antibody | anti-RbAp46/48 antibody

RbAp46/48 antibody (pAb)

Cat. Num. AAA388399

• Gene Names: RBBP7; RbAp46
• Reactivity: Human, Wide Range Predicted
• Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
• Synonyms: RbAp46/48; Polyclonal Antibody; RbAp46/48 antibody (pAb); Retinoblastoma protein associated protein 46/48; anti-RbAp46/48 antibody

• Host: Rabbit
• Reactivity: Human, Wide Range Predicted
• Clonality: Polyclonal
• Form/Format: Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-RbAp46/48 antibody: Chromatin Immunoprecipitation (ChIP), Western Blot (WB)
• Application Notes: Applications Validated:; ChIP: 4ul per ChIP; WB: 1:500-1:2,000 dilution; Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
• Immunogen: This RbAp46/48 antibody was raised against recombinant human RbAp46 protein.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Chromatin Immunoprecipitation (ChIP)

(RbAp46/48 antibody (pAb) tested by ChIP ChIP performed on MCF7 cell chromatin (40 ?g) using 39198. PCR was performed using primers specific for the promoter region of the human SNAIL gene. Lane 1: Input DNA control. Lane 2: ChIP using 4 ?l of 39198. Lane 3: Duplicate. Lane 4: Rabbit IgG as control (-). Lane 5: Duplicate. Lane 6: PCR mix.)

Western Blot (WB)

(RbAp46/48 pAb tested by Western blot. HeLa cell nuclear extract (10 ug per lane) was probed with RbAp46/48 pAb (1:500 dilution).)

Related Product Information for anti-RbAp46/48 antibody

Short Description: Our RbAp46/48 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Background: RbAp46 (Retinoblastoma protein associated protein 46) and RbAp48 (Retinoblastoma protein associated protein 48) are highly homologous histone chaperones that play key roles in protein complexes that function to establish and maintain chromatin structure. RbAp46/48 are found in a number of protein complexes involved in chromatin remodeling (Mi-2b, NURF & NURD), transcriptional repression (Prc2, Sin3) and chromatin assembly (CAF1). Both RbAp46 and RbAp48 are specifically found in the catalytic cores of histone deacetylase (HDAC) complexes that promote transcriptional repression of target genes, including those repressed by the retinoblastoma tumor suppressor protein. RbAp46 binds to and enhances the activity of the type B histone acetyltransferase HAT1, an enzyme that acetylates histone H4 specifically at its Lys5 and Lys12 residues prior to their incorporation into nucleosomes during replication. RbAp48 is an evolutionarily conserved subunit of the chromatin assembly factor-1 (CAF-1) complex, where it associates with two other subunits, known as p150 and p60 in human cells. It is also reported that E2F-1 and RbAp48 are physically associated in the presence of RB1 and histone deacetylase, suggesting that RbAp48 could be involved in transcriptional repression of E2F-responsive genes.

Product Categories/Family for anti-RbAp46/48 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4506439
• NCBI GeneID: 5931
• NCBI Accession #: NP_002884; NP_005601 (RbAp48); AB_2615007
• NCBI GenBank Nucleotide #: NM_002893
• UniProt Accession #: Q16576; Q5JP00
• Molecular Weight: 52,314 Da
• NCBI Official Full Name: histone-binding protein RBBP7 isoform 2
• NCBI Official Synonym Full Names: retinoblastoma binding protein 7
• NCBI Official Symbol: RBBP7
• NCBI Official Synonym Symbols: RbAp46
• NCBI Protein Information: histone-binding protein RBBP7; G1/S transition control protein-binding protein RbAp46; RBBP-7; histone acetyltransferase type B subunit 2; nucleosome-remodeling factor subunit RBAP46; retinoblastoma-binding protein 7; retinoblastoma-binding protein RbAp46
• UniProt Protein Name: Histone-binding protein RBBP7
• UniProt Gene Name: RBBP7
• UniProt Synonym Gene Names: RBAP46; RBBP-7
• UniProt Entry Name: RBBP7_HUMAN