Human Filaggrin ELISA Kit | FLG elisa kit

Human Filaggrin ELISA Kit

Cat. Num. AAA3802844

• Gene Names: FLG; ATOD2
• Reactivity: Human
• Synonyms: Filaggrin; Human Filaggrin ELISA Kit; FLG elisa kit

• Reactivity: Human
• Sequence Length: 4061

• Sensitivity: 10 pg/mL
• Intra-assay Precision: Intra-assay CV is less than 15%.
• Inter-assay Precision: Inter-assay CV is less than 15%.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLG elisa kit

Intended Uses: This FILAGGRIN ELISA kit is intended Laboratory for Research use only. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of FILAGGRIN in the sample, this FILAGGRIN ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus FILAGGRIN concentration. The concentration of FILAGGRIN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 60097902
• NCBI GeneID: 2312
• NCBI Accession #: NP_002007.1
• NCBI GenBank Nucleotide #: NP_002007.1
• UniProt Accession #: P20930
• NCBI Official Full Name: filaggrin
• NCBI Official Synonym Full Names: filaggrin
• NCBI Official Symbol: FLG
• NCBI Official Synonym Symbols: ATOD2
• NCBI Protein Information: filaggrin
• UniProt Protein Name: Friend leukemia integration 1 transcription factor
• Protein Family: Filaggrin
• UniProt Gene Name: FLI1
• UniProt Entry Name: FLI1_HUMAN

NCBI Description

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]

Uniprot Description

FLG: Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. Defects in FLG are the cause of ichthyosis vulgaris (VI); also known as ichthyosis simplex. Ichthyosis vulgaris is the most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever. Defects in FLG are a cause of susceptibility to dermatitis atopic type 2 (ATOD2). Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE- mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. Belongs to the S100-fused protein family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasmic membrane-bound vesicle; intermediate filament; nucleus

Molecular Function: protein binding; calcium ion binding; structural molecule activity

Biological Process: keratinocyte differentiation; multicellular organismal development

Disease: Ichthyosis Vulgaris; Dermatitis, Atopic, 2