Western Blot (WB)
Rabbit anti-Human, mouse CD3 epsilon Antibody | anti-CD3 epsilon antibody
Anti-CD3 epsilon antibody
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Peptide affinity purified
Synonyms
CD3 epsilon; Anti-CD3 epsilon antibody; Polyclonal Anti- CD3 epsilon; anti-CD3 epsilon antibody
Host
Rabbit
Reactivity
Human, mouse
Isotype
IgG
Purity/Purification
Peptide affinity purified
Form/Format
Liquid ;In TBS (pH7.4), 1% BSA, 40% Glycerol and 0.05% Sodium Azide.
Applicable Applications for anti-CD3 epsilon antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC)
Application Notes
Western Blot (1:500)
Immunohistochemistry, IHC (1:100)
Immunocytochemistry, ICC (1:100)
Flow Cytometry, Flow-Cyt (1:50-100)
Other applications have not been tested. The optimal dilutions should be determined by end user.
Immunohistochemistry, IHC (1:100)
Immunocytochemistry, ICC (1:100)
Flow Cytometry, Flow-Cyt (1:50-100)
Other applications have not been tested. The optimal dilutions should be determined by end user.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to the N-terminal of human CD3 epsilon.
Preparation and Storage
Ships on blue ice. after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-CD3 epsilon antibody
In immunology, the CD3 (cluster of differentiation 3) T-cell co-receptor is a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3gamma chain, a CD3delta chain, and two CD3epsilon chains. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3 epsilon, also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11.[1][2] It plays an essential role in T-cell development.
References
1. Gold DP, Puck JM, Pettey CL, Cho M, Coligan J, Woody JN, Terhorst C (1986). "Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex". Nature 321 (6068): 431-4. 2. Clevers HC, Dunlap S, Wileman TE, Terhorst C (November 1988). "Human CD3-epsilon gene contains three miniexons and is transcribed from a non-TATA promoter". Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156-60
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
CD3 epsilon
NCBI Official Symbol
CD3E
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain; CD3E antigen, epsilon polypeptide (TiT3 complex)
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Gene Name
CD3E
UniProt Entry Name
CD3E_CANFA
Uniprot Description
Function: The CD3 complex mediates signal transduction.
Subunit structure: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.
Subcellular location: Membrane; Single-pass type I membrane protein.
Sequence similarities: Contains 1 Ig-like (immunoglobulin-like) domain.Contains 1 ITAM domain.
Research Articles on CD3 epsilon
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Product Notes
The CD3 epsilon cd3e (Catalog #AAA355170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD3 epsilon antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 epsilon can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC). Western Blot (1:500) Immunohistochemistry, IHC (1:100) Immunocytochemistry, ICC (1:100) Flow Cytometry, Flow-Cyt (1:50-100) Other applications have not been tested. The optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the CD3 epsilon cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3 epsilon, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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