Western Blot (WB)
Mouse anti-Human, mouse B-cell lymphoma 2, Bcl-2 Polyclonal Antibody | anti-Bcl-2 antibody
Anti-B-cell lymphoma 2, Bcl-2 antibody
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Protein A or Protein G purified
Synonyms
B-cell lymphoma 2; Bcl-2; Polyclonal Antibody; Anti-B-cell lymphoma 2; Bcl-2 antibody; Monoclonal Anti- B-cell lymphoma 2; anti-Bcl-2 antibody
Host
Mouse
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG2b
Clone Number
D24-5
Purity/Purification
Protein A or Protein G purified
Form/Format
Liquid
Applicable Applications for anti-Bcl-2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes
WB (1:10,000); IHC-P (1:200); ICC (1:500)
Immunogen
Recombinant Bcl-2 protein.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles
Western Blot (WB)
Western Blot (WB)
Testing Data
Testing Data
Related Product Information for anti-Bcl-2 antibody
Bcl-2 (B-cell lymphoma 2) is the founding member of the Bcl-2 family of apoptosis regulator proteins encoded by the BCL2 gene.1,2 Bcl-2 is an important regulator of photoreceptor cell death in retinal degenerations.3 The bcl-2 protooncogene, which protects various cell types from apoptotic cell death, is expressed in the developing and adult nervous system. Bcl-2 may play an important role in survival of neurons both during development and throughout adult life.4
References
1. Tsujimoto Y, Finger LR, Yunis J, Nowell PC, Croce CM (November 1984). "Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation". Science 226 (4678): 1097-99. 2. Cleary ML, Smith SD, Sklar J (October 1986). "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation". Cell 47 (1): 19-28. 3. Chen, J., Flannery, J. G., LaVail, M. M., Steinberg, R. H., Xu, J., Simon, M. I. Bcl-2 overexpression reduces apoptotic photoreceptor cell death in three different retinal degenerations. Proc. Nat. Acad. Sci. 93: 7042-7047, 1996. 4. Farlie, P. G., Dringen, R., Rees, S. M., Kannourakis, G., Bernard, O. bcl-2 transgene expression can protect neurons against developmental and induced cell death. Proc. Nat. Acad. Sci. 92: 4397-4401, 1995.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
bcl-2
Similar Products
Product Notes
The Bcl-2 (Catalog #AAA355094) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-B-cell lymphoma 2, Bcl-2 antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B-cell lymphoma 2, Bcl-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). WB (1:10,000); IHC-P (1:200); ICC (1:500). Researchers should empirically determine the suitability of the Bcl-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B-cell lymphoma 2, Bcl-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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