IL-6 elisa kit

Human IL-6 HS ELISA Kit

Cat. Num. AAA335512

• Reactivity: Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80.
• Synonyms: IL-6; Human IL-6 HS ELISA Kit; IL-6 HS; IL-6 elisa kit

• Reactivity: Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80.
• Specificity: The assay recognizes both natural and recombinant human IL-6. To define the specificity of this ELISA several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1alpha, IL-1beta, IL-10, IL-12, IFNgamma, IL-4, TNFalpha, IL-8 and IL-13).
• Sequence Length: 209

• Samples: Supernatants, buffered solutions or serum and plasma samples and other body fluids. This assay will recognise both natural and recombinant human IL-6.
• Assay Type: Sandwich
• Sensitivity: The sensitivity, minimum detectable dose of IL-6 using this IL-6 HS ELISA kit was found to be less than 0.81pg/ml. This was determined by adding 3 standard deviations to the mean OD obtained when the zero standard was assayed 40 times.
• Intra-assay Precision: Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) of 3 spiked human pooled serum samples and 2 supernatants containing different concentrations of IL-6. Data below shows the mean IL-6 concentration and the coefficient of variation for each sample. The overall intra-assay coefficient of variation has been calculated to be 4.4%.
• Inter-assay Precision: Assay to assay reproducibility within one laboratory was evaluated in three independent experiments by two analysts. Each assay was carried out with 6 replicates of 3 spiked serum human pooled serum samples and 2 supernatants containing different concentrations of IL-6. The calculated overall coefficient of variation was 9.1%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-6 elisa kit

Principle of the Assay: A capture Antibody highly specific for IL-6 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of IL-6 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-IL-6 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IL-6 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IL-6 in any sample tested.

Product Categories/Family for IL-6 elisa kit

Human ELISA Kit

References

Can aerobic exercise alleviate flu-like symptoms following interferon beta-1a injections in patients with multiple sclerosis?. Martin Langeskov-Christensena, Tue Kjølhedea, Egon Stenagerb, Henrik Boye Jensenb, Ulrik Dalgas (2016)

NCBI and Uniprot Product Information

• NCBI GI #: 1161162
• Molecular Weight: 23,483 Da
• NCBI Official Full Name: interleukin 6, partial
• UniProt Protein Name: Interleukin-6
• UniProt Gene Name: IL6
• UniProt Synonym Gene Names: IL-6
• UniProt Entry Name: IL6_PHOVI

Uniprot Description

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().