Western Blot (WB)
(Sample Type: HeLa cell extract, Xenopus laevis egg extractApplication: Western blottingSpecies + Tissue/Cell type: HeLa cell extract 20000 cells/lane, Xenopus laevis egg extract 0.3 ul/laneHow many ug's of tissue/cell lysate run on the gel:1. HeLa cell extract 20000 cells/lane2. Xenopus laevis egg extract 0.3 ul/lanePrimary antibody dilution: 1:1000Secondary antibody: Anti-Rabbit HRPSecondary antibody dilution: 1:5000)
Rabbit TPX2 Polyclonal Antibody | anti-TPX2 antibody
TPX2 antibody - C-terminal region
Western Blot (WB)
(Sample Type: HeLa cell extract, Xenopus laevis egg extractApplication: Western blottingSpecies + Tissue/Cell type: HeLa cell extract 20000 cells/lane, Xenopus laevis egg extract 0.3 ul/laneHow many ug's of tissue/cell lysate run on the gel:1. HeLa cell extract 20000 cells/lane2. Xenopus laevis egg extract 0.3 ul/lanePrimary antibody dilution: 1:1000Secondary antibody: Anti-Rabbit HRPSecondary antibody dilution: 1:5000)
Western Blot (WB)
(Sample Type: HeLa cell extract, Xenopus laevis egg extractApplication: Western blottingSpecies + Tissue/Cell type: HeLa cell extract 20000 cells/lane, Xenopus laevis egg extract 0.3 ul/laneHow many ug's of tissue/cell lysate run on the gel:1. HeLa cell extract 20000 cells/lane2. Xenopus laevis egg extract 0.3 ul/lanePrimary antibody dilution: 1:1000Secondary antibody: Anti-Rabbit HRPSecondary antibody dilution: 1:5000)
Western Blot (WB)
(WB Suggested Anti-TPX2 Antibody Titration: 0.2-1 ug/mlPositive Control: 721_B cell lysateTPX2 is supported by BioGPS gene expression data to be expressed in 721_B)
Western Blot (WB)
(WB Suggested Anti-TPX2 Antibody Titration: 0.2-1 ug/mlPositive Control: 721_B cell lysateTPX2 is supported by BioGPS gene expression data to be expressed in 721_B)
Target Description: TPX2 binding decreases the size and accessibility of a hydrophobic pocket, adjacent to the ATP site, to inhibitors.
NCBI and Uniprot Product Information
Uniprot Description
TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.
Protein type: Microtubule-binding; Cell cycle regulation
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: nucleoplasm; microtubule cytoskeleton; axon hillock; spindle pole; microtubule; cytoplasm; spindle; nucleus
Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding
Biological Process: mitosis; cell proliferation; apoptosis; cell division; activation of protein kinase activity
Research Articles on TPX2
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Product Notes
The TPX2 tpx2 (Catalog #AAA3208098) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TPX2 antibody - C-terminal region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's TPX2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TPX2 tpx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: LSGSLVQEPF QLATEKRAKE RQELEKRMAE VEAQKAQQLE EARLQEEEQK. It is sometimes possible for the material contained within the vial of "TPX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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