Immunohistochemistry (IHC)
(Sample Type :Human lung adenocarcinoma cell line A549Primary Antibody Dilution :1:100Secondary Antibody :Goat anti-rabbit AlexaFluor 488Secondary Antibody Dilution :1:400Color/Signal Descriptions :MAK: Green DAPI:BlueGene Name :MAK Submitted by :Dr. Crissy Dudgeon, Ph.D., CINJ)
Rabbit MAK Polyclonal Antibody | anti-MAK antibody
MAK antibody - C-terminal region
Immunohistochemistry (IHC)
(Sample Type :Human lung adenocarcinoma cell line A549Primary Antibody Dilution :1:100Secondary Antibody :Goat anti-rabbit AlexaFluor 488Secondary Antibody Dilution :1:400Color/Signal Descriptions :MAK: Green DAPI:BlueGene Name :MAK Submitted by :Dr. Crissy Dudgeon, Ph.D., CINJ)
Immunohistochemistry (IHC)
(Sample Type :Human lung adenocarcinoma cell line A549Primary Antibody Dilution :1:100Secondary Antibody :Goat anti-rabbit AlexaFluor 488Secondary Antibody Dilution :1:400Color/Signal Descriptions :MAK: Green DAPI:BlueGene Name :MAK Submitted by :Dr. Crissy Dudgeon, Ph.D., CINJ)
Western Blot (WB)
(Lanes:1. 30 ug MiaPaca-2 cell lysate2. 30 ug Panc-1 cell lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Goat anti-Rabbit HRPSecondary Antibody Dilution:1:4000Gene Name:MAKSubmitted by:Dr. Crissy Dudgeon, Ph.D., CINJ)
Western Blot (WB)
(Lanes:1. 30 ug MiaPaca-2 cell lysate2. 30 ug Panc-1 cell lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Goat anti-Rabbit HRPSecondary Antibody Dilution:1:4000Gene Name:MAKSubmitted by:Dr. Crissy Dudgeon, Ph.D., CINJ)
Western Blot (WB)
(WB Suggested Anti-MAK Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate)
Western Blot (WB)
(WB Suggested Anti-MAK Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate)
Target Description: MAK is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Uniprot Description
MAK: a protein kinase of the RCK family of kinases. It is expressed at high levels in the testis (primarily in germ cells), and in developing sensory epithelia. Localizes to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.22; Nuclear receptor co-regulator; Protein kinase, CMGC; CMGC group; RCK family
Chromosomal Location of Human Ortholog: 6p24
Cellular Component: centrosome; photoreceptor outer segment; photoreceptor inner segment; cytoplasm; midbody; nucleus
Molecular Function: protein binding; cyclin-dependent protein kinase activity; transcription coactivator activity; ATP binding; protein kinase activity
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of cell cycle; multicellular organismal development; protein amino acid autophosphorylation; photoreceptor cell maintenance; spermatogenesis; cell differentiation; protein amino acid phosphorylation
Disease: Retinitis Pigmentosa 62
Research Articles on MAK
Similar Products
Product Notes
The MAK mak (Catalog #AAA3206282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAK antibody - C-terminal region reacts with Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAK can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the MAK mak for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: WNTKTGRGQF SGRTYNPTAK NLNIVNRAQP IPSVHGRTDW VAKYGGHR. It is sometimes possible for the material contained within the vial of "MAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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