Human Apoptosis Inducing Factor ELISA Kit | AIF elisa kit

Human Apoptosis Inducing Factor ELISA Kit

Cat. Num. AAA30770

• Gene Names: AIFM1; AIF; CMTX4; COWCK; PDCD8; COXPD6
• Reactivity: Human
• Synonyms: Apoptosis Inducing Factor; Human Apoptosis Inducing Factor ELISA Kit; AIF elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human AIF and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 6.25ng/ml-200ng/ml.
• Sensitivity: 1.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated AIF concentrations in Human serum, plasma and other body fluids. Using Purified Human AIF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AIF and AIF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AIF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for AIF elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated AIF concentrations in Human serum, plasma and other body fluids. Using Purified Human AIF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AIF and AIF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AIF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55585894
• NCBI GeneID: 9131
• UniProt Accession #: O95831; Q1L6K4; Q1L6K6; Q2QKE4; Q5JUZ7; Q6I9X6; Q9Y3I3; Q9Y3I4; A4QPB4; B1ALN1; B2RB08; D3DTE9
• Molecular Weight: 66,901 Da
• NCBI Official Full Name: apoptosis-inducing factor
• NCBI Official Synonym Full Names: apoptosis-inducing factor, mitochondrion-associated, 1
• NCBI Official Symbol: AIFM1
• NCBI Official Synonym Symbols: AIF; CMTX4; COWCK; PDCD8; COXPD6
• NCBI Protein Information: apoptosis-inducing factor 1, mitochondrial; striatal apoptosis-inducing factor; programmed cell death 8 (apoptosis-inducing factor)
• UniProt Protein Name: Apoptosis-inducing factor 1, mitochondrial
• Protein Family: Putative apoptosis-inducing factor
• UniProt Gene Name: AIFM1
• UniProt Synonym Gene Names: AIF; PDCD8
• UniProt Entry Name: AIFM1_HUMAN

NCBI Description

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]

Uniprot Description

PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Apoptosis; EC 1.-.-.-; Mitochondrial

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; oxidoreductase activity, acting on NADH or NADPH; DNA binding; electron carrier activity; NAD(P)H oxidase activity

Biological Process: chromosome condensation; caspase activation; neuron differentiation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; positive regulation of apoptosis; cell redox homeostasis; apoptosis; DNA fragmentation during apoptosis; DNA catabolic process

Disease: Cowchock Syndrome