Western Blot (WB)
(Western Blot detection of Rad23B in K562,SW480,CHO-K1,3T3 and COS7 cell lysates using Rad23B mouse mAb (1:1000 diluted).)
Mouse Rad23B Monoclonal Antibody | anti-Rad23B antibody
Rad23B (5H1) Monoclonal Antibody
IHC: 1:50-200
ICC: 1:50-200
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot detection of Rad23B in K562,SW480,CHO-K1,3T3 and COS7 cell lysates using Rad23B mouse mAb (1:1000 diluted).)
Western Blot (WB)
(Western Blot detection of Rad23B in K562,SW480,CHO-K1,3T3 and COS7 cell lysates using Rad23B mouse mAb (1:1000 diluted).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Prostate Cancer using Rad23B mouse mAb (1/100 dilution). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6. 0).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Prostate Cancer using Rad23B mouse mAb (1/100 dilution). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6. 0).)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-Rad23B antibody (dilution 1:100).)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-Rad23B antibody (dilution 1:100).)
NCBI and Uniprot Product Information
Observed Band Size: 55KDa
NCBI Description
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]
Uniprot Description
RAD23B: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. Belongs to the RAD23 family.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9q31.2
Cellular Component: nucleoplasm; proteasome complex; cytoplasm; nucleus
Molecular Function: protein binding; damaged DNA binding; polyubiquitin binding; single-stranded DNA binding
Biological Process: nucleotide-excision repair, DNA damage recognition; proteasomal ubiquitin-dependent protein catabolic process; nucleotide-excision repair; regulation of proteasomal ubiquitin-dependent protein catabolic process; spermatogenesis; nucleotide-excision repair, DNA damage removal; DNA repair
Research Articles on Rad23B
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Product Notes
The Rad23B rad23b (Catalog #AAA3014360) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rad23B (5H1) Monoclonal Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Rad23B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). WB: 1:1000 IHC: 1:50-200 ICC: 1:50-200. Researchers should empirically determine the suitability of the Rad23B rad23b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad23B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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