Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Actin-kappa (T121) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Rabbit anti-Human, Mouse Actin-kappa Polyclonal Antibody | anti-T121 antibody
Actin-kappa (T121) Polyclonal Antibody
Gene Names
POTEKP; ACT; POTEK; ACTBL3; POTE2delta
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Actin-kappa; Polyclonal Antibody; Actin-kappa (T121) Polyclonal Antibody; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; FKSG30; ACTBL3; POTEKP; anti-T121 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Actin-kappa (T121) polyclonal antibody detects endogenous levels of Actin-kappa protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
375
Applicable Applications for anti-T121 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 90-134 of Human Actin-kappa.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Actin-kappa (T121) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Actin-kappa (T121) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Actin-kappa (T121) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Actin-kappa (T121) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Related Product Information for anti-T121 antibody
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 42kDa
NCBI Official Full Name
Putative beta-actin-like protein 3
NCBI Official Synonym Full Names
POTE ankyrin domain family member K, pseudogene
NCBI Official Symbol
POTEKP
NCBI Official Synonym Symbols
ACT; POTEK; ACTBL3; POTE2delta
UniProt Protein Name
Putative beta-actin-like protein 3
UniProt Gene Name
POTEKP
UniProt Synonym Gene Names
ACTBL3
Uniprot Description
CautionCould be the product of a pseudogene.
Similar Products
Product Notes
The T121 potekp (Catalog #AAA3013801) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Actin-kappa (T121) Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Actin-kappa can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the T121 potekp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin-kappa, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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