Western Blot (WB)
(Western Blot (WB) analysis of STK33 (N108) pAb at 1:500 dilutionLane1:A2780 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (40ug) Lane4:The Testis tissue lysate of Mouse (40ug) Lane5:HEK293T whole cell lysate (10ug))
Rabbit anti-Human, Mouse STK33 Polyclonal Antibody | anti-STK33 antibody
STK33 (N108) Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
STK33; Polyclonal Antibody; STK33 (N108) Polyclonal Antibody; Serine/threonine-protein kinase 33; anti-STK33 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
STK33 (N108) polyclonal antibody detects endogenous levels of STK33 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
514
Applicable Applications for anti-STK33 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 72-124 of Human STK33.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of STK33 (N108) pAb at 1:500 dilutionLane1:A2780 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (40ug) Lane4:The Testis tissue lysate of Mouse (40ug) Lane5:HEK293T whole cell lysate (10ug))
Western Blot (WB)
(Western Blot (WB) analysis of STK33 (N108) pAb at 1:500 dilutionLane1:A2780 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (40ug) Lane4:The Testis tissue lysate of Mouse (40ug) Lane5:HEK293T whole cell lysate (10ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of STK33 (N108) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of STK33 (N108) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)
Related Product Information for anti-STK33 antibody
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. STK33 (serine/threonine kinase 33) is a 514 amino acid protein that belongs to the CaMK (calcium/calmodulin dependent kinase) subfamily of structurally related serine/threonine kinases. Widely expressed at low levels with predominant expression in testis, lung, retina and fetal organs such as brain, heart and spinal cord, STK33 contains one protein kinase domain and functions as a Ser/Thr protein kinase with a possible role in spermatogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 65kDa
NCBI Official Full Name
serine/threonine-protein kinase 33 isoform a
UniProt Protein Name
Serine/threonine-protein kinase 33
Protein Family
UniProt Gene Name
STK33
UniProt Entry Name
STK33_HUMAN
Uniprot Description
STK33: Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME. Not essential for the survival of KRAS-dependent AML cell lines. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; CAMK-Unique family
Chromosomal Location of Human Ortholog: 11p15.3
Cellular Component: perinuclear region of cytoplasm
Molecular Function: protein serine/threonine kinase activity; ATP binding
Biological Process: protein amino acid autophosphorylation
Similar Products
Product Notes
The STK33 stk33 (Catalog #AAA3013796) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STK33 (N108) Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STK33 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the STK33 stk33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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