Rabbit anti-Human EphA5 Polyclonal Antibody | anti-EphA5 antibody

EphA5 (Y515) Polyclonal Antibody

Cat. Num. AAA3013779

• Gene Names: EPHA5; EK7; CEK7; EHK1; HEK7; EHK-1; TYRO4
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: EphA5; Polyclonal Antibody; EphA5 (Y515) Polyclonal Antibody; Ephrin type-A receptor 5; Brain-specific kinase; EPH homology kinase 1; EHK-1; EPH-like kinase 7; EK7; hEK7; EPHA5; BSK; EHK1; HEK7; TYRO4; anti-EphA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EphA5 (Y515) polyclonal antibody detects endogenous levels of EphA5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1037

• Applicable Applications for anti-EphA5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 500-550 of Human EphA5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of EphA5 (Y515) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:K562 whole cell lysate (40ug))

Related Product Information for anti-EphA5 antibody

The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date. While the biological activities of these receptors have yet to be determined, there is increasing evidence that they are involved in central nervous system function and in development. The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1 (Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8 (Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3 (Cek10, Hek2), EphB4 (Htk), EphB5 (Hek9) and EphB6 (Mep). Ligands for Eph receptors include EphrinA4 (LERK-4) which binds EphA3 and EphB1.

NCBI and Uniprot Product Information

• NCBI GI #: 221625401
• NCBI GeneID: 2044
• NCBI Accession #: NP_004430.4
• NCBI GenBank Nucleotide #: NP_004430.4
• UniProt Accession #: P54756
• Molecular Weight: ~ 130kDa
• NCBI Official Full Name: ephrin type-A receptor 5 isoform a
• NCBI Official Synonym Full Names: EPH receptor A5
• NCBI Official Symbol: EPHA5
• NCBI Official Synonym Symbols: EK7; CEK7; EHK1; HEK7; EHK-1; TYRO4
• NCBI Protein Information: ephrin type-A receptor 5
• UniProt Protein Name: Ephrin type-A receptor 5
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA5
• UniProt Synonym Gene Names: BSK; EHK1; HEK7; TYRO4; EHK-1; EK7; hEK7
• UniProt Entry Name: EPHA5_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]

Uniprot Description

EphA5: Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino- hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 4q13.1

Cellular Component: rough endoplasmic reticulum; cell soma; axon; perinuclear region of cytoplasm; integral to plasma membrane; dendrite; plasma membrane; external side of plasma membrane

Molecular Function: transmembrane-ephrin receptor activity; GPI-linked ephrin receptor activity; ephrin receptor activity; ATP binding

Biological Process: axon guidance; cAMP-mediated signaling; peptidyl-tyrosine phosphorylation; regulation of actin cytoskeleton organization and biogenesis; hippocampus development; activation of CREB transcription factor; ephrin receptor signaling pathway; neuron development