Rabbit anti-Human SAE2/UBA2 Polyclonal Antibody | anti-SAE2 antibody

SAE2/UBA2 Polyclonal Antibody

Cat. Num. AAA3013397

• Gene Names: UBA2; ARX; SAE2; HRIHFB2115
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: SAE2/UBA2; Polyclonal Antibody; SAE2/UBA2 Polyclonal Antibody; UBA2; SAE2; UBLE1B; HRIHFB2115; Anthracycline associated resistance ARX; Anthracycline-associated resistance ARX; ARX; FLJ13058; SAE 2; SUMO 1 activating enzyme subunit 2; SUMO activating enzyme subunit 2; SUMO-activating enzyme subunit 2; UBA2 ubiquitin activating enzyme E1 homolog; Ubiquitin like 1 activating enzyme E1B; Ubiquitin like modifier activating enzyme 2; Ubiquitin-like 1-activating enzyme E1B; anti-SAE2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SAE2/UBA2 polyclonal antibody detects endogenous levels of SAE2/UBA2 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 640

• Applicable Applications for anti-SAE2 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500; IP: 1:10-1:50
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GAB2 pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug))

Related Product Information for anti-SAE2 antibody

The small ubiquitin-related modifier protein SUMO-1 belongs to the ubiquitin-like protein family, which are synthesized as precursor proteins that undergo processing before conjugation to target proteins. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processes, including nuclear transport, transcriptional regulation, apoptosis, and protein stability. SUMO-1 utilizes homologues of the E1 and E2 enzymes for conjugation to proteins, which include IkBalpha, MDM2, p53, PML, and RanGap1. AOS1 is homologous to the N-terminal half of E1 and UBA2 is homologous to the C-terminal half of E1. These proteins form a heterodimer that activates SUMO-1.

NCBI and Uniprot Product Information

• NCBI GI #: 4885649
• NCBI GeneID: 10054
• NCBI Accession #: NP_005490.1
• NCBI GenBank Nucleotide #: NP_005490.1
• UniProt Accession #: Q9UBT2; Q9Z1F9
• Molecular Weight: ~ 80kDa
• NCBI Official Full Name: SUMO-activating enzyme subunit 2
• NCBI Official Synonym Full Names: ubiquitin like modifier activating enzyme 2
• NCBI Official Symbol: UBA2
• NCBI Official Synonym Symbols: ARX; SAE2; HRIHFB2115
• NCBI Protein Information: SUMO-activating enzyme subunit 2
• UniProt Protein Name: SUMO-activating enzyme subunit 2
• Protein Family: DNA endonuclease
• UniProt Gene Name: UBA2
• UniProt Synonym Gene Names: SAE2; UBLE1B
• UniProt Entry Name: SAE2_HUMAN

NCBI Description

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Uniprot Description

SAE2: a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.

Protein type: Ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Motility/polarity/chemotaxis; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: SUMO activating enzyme activity; protein binding; protein heterodimerization activity; metal ion binding; enzyme activator activity; transcription factor binding; ATP binding

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; SMT3-dependent protein catabolic process; post-translational protein modification