Rabbit anti-Human, Mouse S100 Polyclonal Antibody | anti-S100 antibody

S100 Polyclonal Antibody

Cat. Num. AAA3013388

• Gene Names: S100A1; S100; S100A; S100-alpha
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: S100; Polyclonal Antibody; S100 Polyclonal Antibody; S100A1; NEF; Protein S100 B; Protein S100-B; S 100 calcium binding protein beta chain; S 100 protein beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 protein beta chain; S100B; S100beta; anti-S100 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: S100 polyclonal antibody detects endogenous levels of S100 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 94

• Applicable Applications for anti-S100 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; IHC: 1:50-1:200; FC/FACS: 1:50-1:100; IP: 1:10-1:50
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- S100 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-S100 antibody

The family of EF-hand type Ca2+-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 alpha and beta, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin alpha and parvalbumin beta (also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 alpha chain and S-100 beta chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two alpha chains, two beta chains or one alpha and one beta chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 alpha and beta are present in a variety of other tissues, and calbindin is present in intestine and kidney.

NCBI and Uniprot Product Information

• NCBI GI #: 5454032
• NCBI GeneID: 6271
• NCBI Accession #: NP_006262.1
• NCBI GenBank Nucleotide #: NP_006262.1
• UniProt Accession #: P23297; P56565
• Molecular Weight: 11kDa
• NCBI Official Full Name: protein S100-A1
• NCBI Official Synonym Full Names: S100 calcium binding protein A1
• NCBI Official Symbol: S100A1
• NCBI Official Synonym Symbols: S100; S100A; S100-alpha
• NCBI Protein Information: protein S100-A1
• UniProt Protein Name: Protein S100-A1
• Protein Family: S100P-binding protein
• UniProt Gene Name: S100A1
• UniProt Synonym Gene Names: S100A
• UniProt Entry Name: S10A1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.

Protein type: Calcium-binding; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: neuron projection; protein complex; sarcoplasmic reticulum; M band; Z disc; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; calcium ion binding; calcium-dependent protein binding; ATPase binding

Biological Process: substantia nigra development; negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction