Rabbit RPE65 Polyclonal Antibody | anti-RPE65 antibody

RPE65 Polyclonal Antibody

Cat. Num. AAA3013375

• Gene Names: RPE65; p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: RPE65; Polyclonal Antibody; RPE65 Polyclonal Antibody; All-trans-retinyl-palmitate hydrolase; LCA 2; LCA2; Leber congenital amaurosis; mRPE 65; mRPE65; p63; rd 12; rd12; Retinal pigment epithelium specific 61 kDa protein; Retinal pigment epithelium specific 65 kDa protein; Retinal pigment epithelium specific protein; Retinal pigment epithelium specific protein 65kDa; Retinal pigment epithelium-specific 65 kDa protein; Retinitis pigmentosa 20; Retinoid isomerohydrolase; Retinol isomerase; RP 20; RP20; RPE 65; sRPE 65; sRPE65; anti-RPE65 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPE65 polyclonal antibody detects endogenous levels of RPE65 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 533

• Applicable Applications for anti-RPE65 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000-1:2,000; IP: 1:10-1:50
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of RPE65 on mouse eyeball cells lysates using anti-RPE65 antibody at 1/500 dilution.)

Related Product Information for anti-RPE65 antibody

The retinal pigment epithelium (RPE) is a monolayer simple epithelium in proximity to the outer surface of the retinal photoreceptor cells. Retinal pigment epithelium-specific protein (RPE65) is a 65kDa protein belonging to the beta-carotene dioxygenase family. This protein is important in 11-cis retinal production as well as in visual pigment regeneration. RPE65 is attached to the membrane by a lipid anchor when palmitoylated (membrane form) and soluble when unpalmitoylated. The soluble form of the protein binds vitamin A. Defects in RPE65 causes autosomal dominant retinitis pigmentosa and/or Leber congenital amaurosis type 2.

NCBI and Uniprot Product Information

• NCBI GI #: 4506591
• NCBI GeneID: 6121
• NCBI Accession #: NP_000320.1
• NCBI GenBank Nucleotide #: NP_000320.1
• UniProt Accession #: Q16518; Q91ZQ5; O70276
• Molecular Weight: 61kDa
• NCBI Official Full Name: retinoid isomerohydrolase
• NCBI Official Synonym Full Names: retinoid isomerohydrolase RPE65
• NCBI Official Symbol: RPE65
• NCBI Official Synonym Symbols: p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65
• NCBI Protein Information: retinoid isomerohydrolase
• UniProt Protein Name: Retinoid isomerohydrolase
• UniProt Gene Name: RPE65

NCBI Description

The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017]

Uniprot Description

Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:16116091). Essential for the production of 11-cis retinal for both rod and cone photoreceptors (PubMed:17848510). Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid (PubMed:28874556). The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT ().