Western Blot (WB)
(Western Blot analysis of Human IgM on human plasma lysates using anti-Human IgM antibody at 1/1,000 dilution.)
Rabbit anti-Human IgM Polyclonal Antibody | anti-IgM antibody
Human IgM Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
IgM; Polyclonal Antibody; Human IgM Polyclonal Antibody; IGHM; Immunoglobin heavy chain constant region mu edit item name - Immunoglobin heavy chain mu constant region; Immunoglobin heavy chain constant region mu; AGM1; Constant region of heavy chain of IgM; DKFZp686I15196; DKFZp686I15212; FLJ00385; Ig mu chain C region; IgM heavy chain constant region; Immunoglobin heavy constant mu; Immunoglobulin mu; MGC104996; MGC52291; MU; VH; anti-IgM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human IgM polyclonal antibody detects endogenous levels of Human IgM protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
452
Applicable Applications for anti-IgM antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:1,000-1:5,000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Human IgM on human plasma lysates using anti-Human IgM antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of Human IgM on human plasma lysates using anti-Human IgM antibody at 1/1,000 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Human IgM antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Human IgM antibody. Counter stained with hematoxylin.)
Related Product Information for anti-IgM antibody
Immunoglobulin M (IgM) is the largest circulating antibody molecule in humans. It consists of a heavy chain (-chain) and a light chain (kappa- or lambda-chain), as well as 5 base units and 10 binding sites, though it cannot bind all 10 simultaneously because of steric hindrance. IgM chain C refers to the constant region of the IgM heavy chain that is involved in immune regulation. IgM forms polymers by covalently linking multiple immunoglobulins together with disulfide bonds. It normally exists as a pentamer, but occasionally as a hexamer. Because of its polymeric nature, IgM has high avidity, and it is especially effective at complement activation. Due to its large size, IgM does not diffuse well, and it is found in the interstitium in very low amounts. IgM is mainly found in serum; however, because of the J chain, it is also important as a secretory immunoglobulin. IgM is the first immunoglobulin expressed by mature B cells, and it normally appears early in the course of an infection and does not reappear after further exposure.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
75kDa
NCBI Official Full Name
Ig mu chain C region
UniProt Protein Name
Ig mu chain C region
Protein Family
UniProt Gene Name
IGHM
UniProt Entry Name
IGHM_HUMAN
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Product Notes
The IgM ighm (Catalog #AAA3012861) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Human IgM Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1,000-1:5,000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the IgM ighm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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