Western Blot (WB)
(Western Blot analysis of CTDSPL on HepG2 cell lysate using anti-CTDSPL antibody at 1/500 dilution.)
Rabbit CTDSPL Polyclonal Antibody | anti-CTDSPL antibody
CTDSPL Polyclonal Antibody
Gene Names
CTDSPL; PSR1; SCP3; HYA22; RBSP3; C3orf8
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Protein affinity purified.
Synonyms
CTDSPL; Polyclonal Antibody; CTDSPL Polyclonal Antibody; CTDSP-like; NLI-interacting factor 1; hYA22; SCP3; Small CTD phosphatase 3; C3orf8; NIF1; NIFL; YA22; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3; CTD (carboxy-terminal domain; RNA polymerase II; polypeptide A) small phosphatase-like; CTD small phosphatase-like protein; CTDSL; Ctdspl; N-like protein; NIF-like protein; Nuclear LIM interactor-interacting factor 1; Protein YA22; RB protein serine phosphatase from chromosome 3; RBSP3; Small C-terminal domain phosphatase 3; anti-CTDSPL antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CTDSPL polyclonal antibody detects endogenous levels of CTDSPL protein.
Purity/Purification
Protein affinity purified.
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
276
Applicable Applications for anti-CTDSPL antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
FC/FACS: 1:50-1:100
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein within human CTDSPL aa 50-300.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of CTDSPL on HepG2 cell lysate using anti-CTDSPL antibody at 1/500 dilution.)
Western Blot (WB)
(Western Blot analysis of CTDSPL on HepG2 cell lysate using anti-CTDSPL antibody at 1/500 dilution.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells with CFTR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells with CFTR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-CTDSPL antibody
Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity). Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31kDa
NCBI Official Full Name
CTD small phosphatase-like protein isoform 1
NCBI Official Synonym Full Names
CTD small phosphatase like
NCBI Official Symbol
CTDSPL
NCBI Official Synonym Symbols
PSR1; SCP3; HYA22; RBSP3; C3orf8
NCBI Protein Information
CTD small phosphatase-like protein
UniProt Protein Name
CTD small phosphatase-like protein
Protein Family
UniProt Gene Name
CTDSPL
UniProt Synonym Gene Names
C3orf8; NIF1; NIFL; SCP3; YA22; CTDSP-like; NLI-interacting factor 1; hYA22; SCP3; Small CTD phosphatase 3
UniProt Entry Name
CTDSL_HUMAN
Uniprot Description
CTDSPL: Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.16; Phosphatase
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: nucleus
Molecular Function: metal ion binding; phosphoprotein phosphatase activity
Biological Process: negative regulation of protein amino acid phosphorylation; dephosphorylation
Research Articles on CTDSPL
Similar Products
Product Notes
The CTDSPL ctdspl (Catalog #AAA3012518) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CTDSPL Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CTDSPL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the CTDSPL ctdspl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTDSPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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