Western Blot (WB)
(Western Blot analysis of CDC5L on different lysates using anti-CDC5L antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat)
Rabbit CDC5L Polyclonal Antibody | anti-CDC5L antibody
CDC5L Polyclonal Antibody
ICC: 1:50-1:200
IHC: 1:50-1:200
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of CDC5L on different lysates using anti-CDC5L antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat)
Western Blot (WB)
(Western Blot analysis of CDC5L on different lysates using anti-CDC5L antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat)
Immunocytochemistry (ICC)
(ICC staining CDC5L in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining CDC5L in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008]
Uniprot Description
CDC5L: is a spliceosome protein that binds DNA and that also regulates transcription. Is a positive regulator of cell cycle G2/M progression. Is an essential component of non-snRNA spliceosomes and is required for the second catalytic step of pre-mRNA splicing. Binds adeno-pre-mRNA in an ATP-stimulated manner. Is part of a spliceosomal 'core' complex that includes CDC5L, SF2, PRLI, SPF27, HSP7C, PRPF19/SNEV, GCN1L. Identified in the spliceosome C complex. Has been associated with aneuploidy in aging cells and in tumorigenesis. The genomic region containing the gene for CDC5L (6p21.1) is frequently amplified in osteosarcomas.
Protein type: DNA-binding; RNA processing; Transcription factor; Spliceosome; Motility/polarity/chemotaxis; RNA splicing
Chromosomal Location of Human Ortholog: 6p21
Cellular Component: nucleoplasm; DNA replication factor A complex; membrane; cytoplasm; nucleolus; nuclear speck; nucleus
Molecular Function: protein binding; DNA binding; chromatin binding
Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; gene expression; DNA repair
Research Articles on CDC5L
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Product Notes
The CDC5L cdc5l (Catalog #AAA3012453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC5L Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDC5L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1,000 ICC: 1:50-1:200 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CDC5L cdc5l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC5L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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