Western Blot (WB)
(Western Blot analysis of BMAL1 on SYSH5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution.)
Rabbit BMAL1 Polyclonal Antibody | anti-BMAL1 antibody
BMAL1 Polyclonal Antibody
Gene Names
ARNTL; TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein affinity purified.
Synonyms
BMAL1; Polyclonal Antibody; BMAL1 Polyclonal Antibody; bHLHe5; ARNTL; BHLHE5; MOP3; PASD3; ARNT like protein 1 brain and muscle; Arntl; Aryl hydrocarbon receptor nuclear translocator like; Aryl hydrocarbon receptor nuclear translocator like protein 1; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic helix loop helix PAS orphan MOP3; Basic helix loop helix PAS protein MOP3; Basic-helix-loop-helix-PAS protein MOP3; bHLH PAS protein JAP3; bHLH-PAS protein JAP3; BMAL 1; BMAL1c; Brain and muscle ARNT like 1; Brain and muscle ARNT-like 1; CG8727 PA; Class E basic helix-loop-helix protein 5; cycle; JAP 3; JAP3; Member of PAS protein 3; Member of PAS superfamily 3; MGC47515; MOP 3; PAS domain-containing protein 3; PASD 3; TIC; anti-BMAL1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
BMAL1 polyclonal antibody detects endogenous levels of BMAL1 protein.
Purity/Purification
Protein affinity purified.
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
625
Applicable Applications for anti-BMAL1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:100-1:500
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of BMAL1 on SYSH5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of BMAL1 on SYSH5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution.)
Immunocytochemistry (ICC)
(ICC staining BMAL1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining BMAL1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-BMAL1 antibody
BMAL1, ARNTL2 (aryl hydrocarbon receptor nuclear translocator-like 2) is a basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor. ARNTL2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription. Also, in umbilical vein endothelial cells, ARNTL2 activates SERPINE1 through E-box sites. This transactivation is inhibited by PER2 and CRY1. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA, F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina. The CLOCK-ARNTL/BMAL1 heterodimer also recognizes the non-canonical E-box motifs 5'-AACGTGA-3' and 5'-CATGTGA-3'. Essential for the rhythmic interaction of CLOCK with ASS1 and plays a critical role in positively regulating CLOCK-mediated acetylation of ASS1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68kDa
NCBI Official Full Name
aryl hydrocarbon receptor nuclear translocator-like protein 1 isoform a
NCBI Official Synonym Full Names
aryl hydrocarbon receptor nuclear translocator like
NCBI Official Symbol
ARNTL
NCBI Official Synonym Symbols
TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
NCBI Protein Information
aryl hydrocarbon receptor nuclear translocator-like protein 1
UniProt Protein Name
Aryl hydrocarbon receptor nuclear translocator-like protein 1
UniProt Gene Name
ARNTL
UniProt Synonym Gene Names
BHLHE5; BMAL1; MOP3; PASD3; bHLHe5
UniProt Entry Name
BMAL1_HUMAN
NCBI Description
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Research Articles on BMAL1
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Product Notes
The BMAL1 arntl (Catalog #AAA3012311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BMAL1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BMAL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-2,000 ICC: 1:100-1:500 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the BMAL1 arntl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMAL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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