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Western Blot (WB) (Western Blot (WB) analysis of PLA2G2D polyclonal antibody)

Rabbit PLA2G2D Polyclonal Antibody | anti-PLA2G2D antibody

PLA2G2D Polyclonal Antibody

Gene Names
PLA2G2D; SPLASH; sPLA2S; PLA2IID; sPLA2-IID
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PLA2G2D; Polyclonal Antibody; PLA2G2D Polyclonal Antibody; Group IID secretory phospholipase A2; GIID sPLA2; sPLA2-IID; PLA2IID; Phosphatidylcholine 2-acylhydrolase 2D; Secretory-type PLA; stroma-associated homolog; SPLASH; anti-PLA2G2D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PLA2G2D polyclonal antibody detects endogenous levels of PLA2G2D protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
145
Applicable Applications for anti-PLA2G2D antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
Recombinant full length Human PLA2G2D.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PLA2G2D polyclonal antibody)

Western Blot (WB) (Western Blot (WB) analysis of PLA2G2D polyclonal antibody)
Related Product Information for anti-PLA2G2D antibody
Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids, many of which are potent mediators of inflammation. As a member of the PLA2 family, group IID sPLA2, also known as PLA2G2D (phosphatidylcholine 2-acylhydrolase 2D), sPLA2-IID or SPLASH, is a 145 amino acid secreted protein that is broadly expressed but found at highest levels in pancreas and spleen. Group IID sPLA2 catalyzes the reaction of phosphatidylcholine and water into 1-acylglycerophosphocholine and carboxylate, and has been liked to body weight loss in patients with chronic obstructive pulmonary disease (COPD), as well as lymphotoxin deficiency.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 17kDa
NCBI Official Full Name
Group IID secretory phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2 group IID
NCBI Official Symbol
PLA2G2D
NCBI Official Synonym Symbols
SPLASH; sPLA2S; PLA2IID; sPLA2-IID
NCBI Protein Information
group IID secretory phospholipase A2
UniProt Protein Name
Group IID secretory phospholipase A2
UniProt Gene Name
PLA2G2D
UniProt Synonym Gene Names
SPLASH; GIID sPLA2; sPLA2-IID
UniProt Entry Name
PA2GD_HUMAN

NCBI Description

This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

Uniprot Description

PLA2G2D: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2- linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Secreted; Lipid Metabolism - glycerophospholipid; Secreted, signal peptide; Phospholipase; EC 3.1.1.4; Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: extracellular region

Molecular Function: phospholipase A2 activity; calcium ion binding

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; inflammatory response; lipid catabolic process

Research Articles on PLA2G2D

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Product Notes

The PLA2G2D pla2g2d (Catalog #AAA3011674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLA2G2D Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G2D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PLA2G2D pla2g2d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLA2G2D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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