PLA2G2D recombinant protein

PLA2G2D Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121177

• Gene Names: PLA2G2D; SPLASH; sPLA2S; PLA2IID; sPLA2-IID
• Purity: >85% as determined by SDS-PAGE
• Synonyms: PLA2G2D; PLA2G2D Protein; Human; Recombinant (hFc Tag); Human PLA2G2D/Phospholipase A2 IID Protein (Fc Tag); PLA2IID Protein; sPLA2-IID Protein; sPLA2S Protein; SPLASH Protein; phospholipase A2; group IID; PLA2G2D recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys145

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 21
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human PLA2G2D (Q9UNK4)(Met1-Cys145) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 20139286
• NCBI GeneID: 26279
• UniProt Accession #: Q9UNK4; Q9UK01; A8K2Z1; B1AEL9
• Molecular Weight: 145
• NCBI Official Full Name: Group IID secretory phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group IID
• NCBI Official Symbol: PLA2G2D
• NCBI Official Synonym Symbols: SPLASH; sPLA2S; PLA2IID; sPLA2-IID
• NCBI Protein Information: group IID secretory phospholipase A2; GIID sPLA2; secretory phospholipase A2s; phosphatidylcholine 2-acylhydrolase 2D; secretory-type PLA, stroma-associated homolog
• UniProt Protein Name: Group IID secretory phospholipase A2
• Protein Family: Group IID secretory phospholipase
• UniProt Gene Name: PLA2G2D
• UniProt Synonym Gene Names: SPLASH; GIID sPLA2; sPLA2-IID
• UniProt Entry Name: PA2GD_HUMAN

NCBI Description

This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

Uniprot Description

PLA2G2D: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2- linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Secreted; Lipid Metabolism - glycerophospholipid; Secreted, signal peptide; Phospholipase; EC 3.1.1.4; Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: extracellular region

Molecular Function: phospholipase A2 activity; calcium ion binding

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; inflammatory response; lipid catabolic process