Rabbit KLK4 Polyclonal Antibody | anti-KLK4 antibody
KLK4 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
KLK4; Polyclonal Antibody; KLK4 Polyclonal Antibody; Kallikrein-4; Enamel matrix serine proteinase 1; Kallikrein-like protein 1; KLK-L1; Prostase; Serine protease 17; EMSP1; PRSS17; PSTS; anti-KLK4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
KLK4 polyclonal antibody detects endogenous levels of KLK4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
254
Applicable Applications for anti-KLK4 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human KLK4.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using KLK4 Antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cell using KLK4 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-KLK4 antibody
Kallikreins (KLKs) belong to the serine protease family of proteolytic enzymes. Human pancreatic/renal KLK encodes for the KLK1 enzyme, which is involved in post-translational processing of polypeptide precursors. The function of the other members of KLK gene family is currently unknown, but evidence suggests that many KLKs are implicated in carcinogenesis. The human KLK gene family consists of 15 serine proteases. The human KLK genes are clustered on chromosome 19q13. Unlike other kalllikreins, the KLK4-15 encoded proteases are less related and do not contain a conventional KLK loop. Clusters of genes exhibit high prostatic (KLK2-4, KLK15) or pancreatic (KLK6-13) expression. KLK2 is also known as glandular kallikrein 2, tissue kallikrein, or HGK-1 and KLK3 is known as prostate-specific antigen (PSA). Both KLK2 and KLK3 have important applications in prostate cancer and breast cancer tests. KLK4, KLK5, KLK9, KLK13, KLK12 and KLK14 have been previously known as KLK-L1, KLK-L2, KLK-L3, KLK-L4, KLK-L5 and KLK-L6, respectively. Many of the KLKs are regulated by steroid hormones and a few of them, specifically KLK3, KLK6 and KLK10, are known to be downregulated in breast and other cancers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 27kDa
NCBI Official Full Name
kallikrein-4 isoform 1 preproprotein
UniProt Protein Name
Kallikrein-4
Protein Family
UniProt Gene Name
KLK4
UniProt Synonym Gene Names
EMSP1; PRSS17; PSTS; KLK-L1
UniProt Entry Name
KLK4_HUMAN
Uniprot Description
KLK4: Involved in enamel formation. Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1). AI2A1 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase S1 family. Kallikrein subfamily.
Protein type: EC 3.4.21.-; Protease; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: extracellular region
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; metal ion binding
Biological Process: extracellular matrix disassembly; protein catabolic process; proteolysis
Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia1
