Rabbit SNRPA Polyclonal Antibody | anti-SNRPA antibody

SNRPA Polyclonal Antibody

Cat. Num. AAA3011555

• Gene Names: SNRPA; U1A; Mud1; U1-A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SNRPA; Polyclonal Antibody; SNRPA Polyclonal Antibody; U1 small nuclear ribonucleoprotein A; U1 snRNP A; U1-A; U1A; anti-SNRPA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SNRPA polyclonal antibody detects endogenous levels of SNRPA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 282

• Applicable Applications for anti-SNRPA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human SNRPA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SNRPA polyclonal antibody)

Related Product Information for anti-SNRPA antibody

SNRPA (small nuclear ribonucleoprotein polypeptide A), also known as U1A (U1 snRNP protein A), is a component of the RNA spliceosome, a complex of proteins that are required for the precise excision of introns from pre-messenger RNA (pre-mRNA). Localizing to the nucleus, SNRPA contains two RRM (RNA recognition motif) domains, namely RRM1 and RRM2, and RRM1 specifically associates with the stem loop II of U1 snRNA (small nuclear RNA). In addition to functioning as a component of the U1 snRNP, SNRPA negatively regulates polyadenylation of SNRPA pre-mRNA, thereby negatively regulating itself. By inhibiting the addition of a polyA tail that would allow the pre-mRNA to mature, SNRPA causes the nuclear exosome degradation of the SNRPA pre-mRNA. At least 16% of cellular SNRPA also functions in an snRNP-free form (SF-A) that complexes with a group of non-snRNP proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 4759156
• NCBI GeneID: 6626
• NCBI Accession #: NP_004587.1
• NCBI GenBank Nucleotide #: NP_004587.1
• UniProt Accession #: P09012
• Molecular Weight: ~ 31kDa
• NCBI Official Full Name: U1 small nuclear ribonucleoprotein A
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein polypeptide A
• NCBI Official Symbol: SNRPA
• NCBI Official Synonym Symbols: U1A; Mud1; U1-A
• NCBI Protein Information: U1 small nuclear ribonucleoprotein A
• UniProt Protein Name: U1 small nuclear ribonucleoprotein A
• Protein Family: U1 small nuclear ribonucleoprotein
• UniProt Gene Name: SNRPA
• UniProt Synonym Gene Names: U1 snRNP A; U1-A; U1A
• UniProt Entry Name: SNRPA_HUMAN

NCBI Description

The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010]

Uniprot Description

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.