Rabbit MAP1LC3A Polyclonal Antibody | anti-MAP1LC3A antibody

MAP1LC3A Polyclonal Antibody

Cat. Num. AAA3011213

• Gene Names: MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAP1LC3A; Polyclonal Antibody; MAP1LC3A Polyclonal Antibody; ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3 like protein 1; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; Map1lc3a; Microtubule associated proteins 1A/1B light chain 3; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A_HUMAN; anti-MAP1LC3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAP1LC3A polyclonal antibody detects endogenous levels of MAP1LC3A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 121

• Applicable Applications for anti-MAP1LC3A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human MAP1LC3A.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MAP1LC3A polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of MAP1LC3A polyclonal antibody in paraffin-embedded Human Kidney Cancer tissue.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells, using MAP1LC3A polyclonal antibody)

Related Product Information for anti-MAP1LC3A antibody

Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy.

NCBI and Uniprot Product Information

• NCBI GI #: 85701362
• NCBI GeneID: 84557
• UniProt Accession #: Q9H492
• Molecular Weight: ~14kDa
• NCBI Official Full Name: Microtubule-associated proteins 1A/1B light chain 3A
• NCBI Official Synonym Full Names: microtubule associated protein 1 light chain 3 alpha
• NCBI Official Symbol: MAP1LC3A
• NCBI Official Synonym Symbols: LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3A
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3A
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3A
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 A
• UniProt Entry Name: MLP3A_HUMAN

NCBI Description

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Uniprot Description

LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Ubiquitin-like modifier; Autophagy; Vesicle

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding

Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation