Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using CYLD antibody at 1:1000 dilution.)
Rabbit CYLD Polyclonal Antibody | anti-CYLD antibody
CYLD Polyclonal Antibody
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using CYLD antibody at 1:1000 dilution.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using CYLD antibody at 1:1000 dilution.)
NCBI and Uniprot Product Information
NCBI Description
This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
CYLD: Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis. Interacts (via CAP-Gly domain) with IKBKG/NEMO (via proline-rich C-terminal region). Interacts with TRAF2 and TRIP. Interacts with PLK1, DVL1, DVL3, MAVS, TBK1, IKKE and DDX58. Interacts (via CAP-Gly domain) with microtubules. Interacts with HDAC6 and BCL3. Interacts with SQSTM1 and MAP3K7. Identified in a complex with TRAF6 and SQSTM1. Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney. Inhibited by phosphorylation at serine residues. Belongs to the peptidase C67 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin-specific protease; Protease; Tumor suppressor; EC 3.4.19.12; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 16q12.1
Cellular Component: nucleoplasm; centrosome; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasmic microtubule; spindle; midbody; cytosol
Molecular Function: protein binding; structural constituent of ribosome; zinc ion binding; ubiquitin-specific protease activity; protein kinase binding
Biological Process: ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; inhibition of NF-kappaB transcription factor; regulation of mitotic cell cycle; innate immune response; negative regulation of NF-kappaB import into nucleus; cell cycle; negative regulation of interferon type I production; negative regulation of T cell differentiation
Disease: Brooke-spiegler Syndrome; Cylindromatosis, Familial; Trichoepithelioma, Multiple Familial, 1
Research Articles on CYLD
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Product Notes
The CYLD cyld (Catalog #AAA3009961) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYLD Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CYLD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200-1:2000. Researchers should empirically determine the suitability of the CYLD cyld for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYLD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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