Rabbit CARP Polyclonal Antibody | anti-CARP antibody

CARP Polyclonal Antibody

Cat. Num. AAA3009889

• Gene Names: ANKRD1; ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CARP; Polyclonal Antibody; CARP Polyclonal Antibody; Ankyrin repeat domain-containing protein 1; Cardiac ankyrin repeat protein; Cytokine-inducible gene C-193 protein; Cytokine-inducible nuclear protein; ANKRD1; C193; HA1A2; anti-CARP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CARP polyclonal antibody detects endogenous levels of CARP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 319

• Applicable Applications for anti-CARP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human ANKRD1
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using ANKRD1 antibody at 1:1000 dilution.)

Related Product Information for anti-CARP antibody

A proposed genetic marker of cardiac hypertrophy, CARP (cardiac ankyrin repeat protein, ANKRD1, Ankyrin repeat domain-containing protein 1, ) is a nuclear protein with an established role in regulation of cardiac gene expression. A distinct increase in CARP expression occurs in rats with abdominal aorta constriction, spontaneous hypertension and Dahl salt-sensitivity. In cardiomyocytes, CARP inhibits transcription of both cardiac troponin C and atrial natriuretic factor. Specifically, expression of the CARP gene, which lies downstream of the cardiac homeobox gene Nkx2.5, inhibits Nkx2.5 transactivation of atrial natriuretic factor promoter. An increase in CARP expression is observed in the ventricular tissue of patients with endstage heart failure.

NCBI and Uniprot Product Information

• NCBI GI #: 38327522
• NCBI GeneID: 27063
• NCBI Accession #: NP_055206.2
• NCBI GenBank Nucleotide #: NP_055206.2
• UniProt Accession #: Q15327
• Molecular Weight: 36kDa
• NCBI Official Full Name: ankyrin repeat domain-containing protein 1
• NCBI Official Synonym Full Names: ankyrin repeat domain 1
• NCBI Official Symbol: ANKRD1
• NCBI Official Synonym Symbols: ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
• NCBI Protein Information: ankyrin repeat domain-containing protein 1
• UniProt Protein Name: Ankyrin repeat domain-containing protein 1
• UniProt Gene Name: ANKRD1
• UniProt Synonym Gene Names: C193; CARP; HA1A2
• UniProt Entry Name: ANKR1_HUMAN

NCBI Description

The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor. Interactions between this protein and the sarcomeric proteins myopalladin and titin suggest that it may also be involved in the myofibrillar stretch-sensor system. [provided by RefSeq, Jul 2008]

Uniprot Description

ANKRD1: May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells. Defects in ANKRD1 may be a cause of total anomalous pulmonary venous return (TAPVR). TAPVR is a rare congenital heart disease (CHD) in which the pulmonary veins fail to connect to the left atrium during cardiac development, draining instead into either the right atrium or one of its venous tributaries. This disease accounts for 1.5% of all CHDs and has a prevalence of approximately 1 out of 15'000 live births.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 10q23.31

Cellular Component: nucleoplasm; I band; transcription factor complex; sarcomere; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein binding; DNA binding; p53 binding; histone deacetylase binding; titin binding; transcription corepressor activity

Biological Process: positive regulation of protein secretion; positive regulation of apoptosis; cardiac muscle morphogensis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; sarcomere organization