Rabbit AAK1 Polyclonal Antibody | anti-AAK1 antibody

AAK1 Antibody

Cat. Num. AAA9505275

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: AAK1; Polyclonal Antibody; AAK1 Antibody; KIAA1048; kinase AAK1; anti-AAK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: AAK1 Antibody detects endogenous levels of total AAK1 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 863

• Applicable Applications for anti-AAK1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human AAK1.
• Immunogen Range: 271-320
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from COS7 cells, using AAK1 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 148277037
• NCBI GeneID: 22848
• NCBI Accession #: NP_055726.3
• NCBI GenBank Nucleotide #: NM_014911.3
• UniProt Accession #: Q2M2I8; Q4ZFZ3; Q53RX6; Q9UPV4
• Molecular Weight: 93 kDa
• NCBI Official Full Name: AP2-associated protein kinase 1
• NCBI Official Synonym Full Names: AP2 associated kinase 1
• NCBI Official Symbol: AAK1
• NCBI Protein Information: AP2-associated protein kinase 1
• UniProt Protein Name: AP2-associated protein kinase 1
• Protein Family: AP2-associated protein kinase
• UniProt Gene Name: AAK1
• UniProt Synonym Gene Names: KIAA1048

NCBI Description

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.