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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse lung using PHF11 antibody at dilution of 1:100 (x400 lens).)

Rabbit PHF11 Polyclonal Antibody | anti-PHF11 antibody

PHF11 Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PHF11; Polyclonal Antibody; PHF11 Polyclonal Antibody; APY; BCAP; IGEL; IGER; IGHER; NYREN34; NY-REN-34; anti-PHF11 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PHF11 polyclonal antibody detects endogenous levels of PHF11 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
331
Applicable Applications for anti-PHF11 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:100
Immunogen
Recombinant protein of human PHF11.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse lung using PHF11 antibody at dilution of 1:100 (x400 lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse lung using PHF11 antibody at dilution of 1:100 (x400 lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using PHF11 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using PHF11 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using PHF11 antibody.)

Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using PHF11 antibody.)
Related Product Information for anti-PHF11 antibody
PHF11 (PHD Finger Protein 11A), also known as Phf11a, is a 293 amino acid member of the PHD finger protein family. Members of the PHD finger protein family function as transcriptional regulators that affect gene expression by modulating chromatin structure. With a subcellular localization to the nucleus, PHF11 is expressed in heart, kidney, liver spleen, testis and ureter tissues. PFH11 contains one PHD-type zinc finger domain. PHF11 is thought to interact with HDAC9. It is also thought that PHF11 may be involved in the immune response through regulation of T cell activities in humans, and may operate as a Th1 cell regulator in immune responses in mice. The PHF11 gene is conserved in human, chimpanzee, canine, bovine, rat, and chicken.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
~ 38kDa
NCBI Official Full Name
PHD finger protein 11
UniProt Protein Name
PHD finger protein 11
Protein Family
UniProt Gene Name
PHF11
UniProt Synonym Gene Names
BCAP
UniProt Entry Name
PHF11_HUMAN

Uniprot Description

PHF11: Positive regulator of Th1-type cytokine gene expression. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: nucleus

Molecular Function: zinc ion binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent

Disease: Asthma, Susceptibility To; Ige Responsiveness, Atopic

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Product Notes

The PHF11 phf11 (Catalog #AAA3008884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHF11 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PHF11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:100. Researchers should empirically determine the suitability of the PHF11 phf11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHF11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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