Rabbit anti-Human, Mouse RHOB Polyclonal Antibody | anti-RHOB antibody

RHOB Polyclonal Antibody

Cat. Num. AAA3008486

• Gene Names: RHOB; ARH6; ARHB; RHOH6; MST081; MSTP081
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RHOB; Polyclonal Antibody; RHOB Polyclonal Antibody; ARH6; ARHB; RHOH6; MST081; MSTP081; anti-RHOB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RHOB polyclonal antibody detects endogenous levels of RHOB protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 196

• Applicable Applications for anti-RHOB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide of human RHOB.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using RHOB antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gastric using RHOB antibody at dilution of 1:100 (x40 lens).)

Related Product Information for anti-RHOB antibody

The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

NCBI and Uniprot Product Information

• NCBI GI #: 4757764
• NCBI GeneID: 388
• NCBI Accession #: NP_004031.1
• NCBI GenBank Nucleotide #: NP_004031.1
• UniProt Accession #: P62745
• Molecular Weight: ~ 22kDa
• NCBI Official Full Name: rho-related GTP-binding protein RhoB
• NCBI Official Synonym Full Names: ras homolog family member B
• NCBI Official Symbol: RHOB
• NCBI Official Synonym Symbols: ARH6; ARHB; RHOH6; MST081; MSTP081
• NCBI Protein Information: rho-related GTP-binding protein RhoB
• UniProt Protein Name: Rho-related GTP-binding protein RhoB
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOB
• UniProt Synonym Gene Names: ARH6; ARHB; h6
• UniProt Entry Name: RHOB_HUMAN

Uniprot Description

RHOB: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: Tumor suppressor; G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis; G protein

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: focal adhesion; late endosome membrane; early endosome; plasma membrane; endosome membrane; nucleus; cytosol; cleavage furrow

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: axon guidance; platelet activation; transformed cell apoptosis; metabolic process; apoptosis; positive regulation of apoptosis; cytokinesis; Rho protein signal transduction; negative regulation of cell cycle; intracellular protein transport; positive regulation of angiogenesis; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; endosome to lysosome transport; angiogenesis; blood coagulation; cell adhesion