Rabbit anti-Human DDX58 Polyclonal Antibody | anti-DDX58 antibody

DDX58 Polyclonal Antibody

Cat. Num. AAA3008221

• Gene Names: DDX58; RIG1; RIGI; RIG-I; RLR-1; SGMRT2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DDX58; Polyclonal Antibody; DDX58 Polyclonal Antibody; Probable ATP-dependent RNA helicase DDX58; DEAD box protein 58; RIG-I-like receptor 1; RLR-1; RLR1; Retinoic acid-inducible gene 1 protein; RIG-1; RIG1; Retinoic acid-inducible gene I protein; RIG-I; RIGI; anti-DDX58 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DDX58 polyclonal antibody detects endogenous levels of DDX58 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 925

• Applicable Applications for anti-DDX58 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:20-1:100; IP: 1:20-1:100
• Immunogen: Recombinant full length Human DDX58.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DDX58 pAb)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of DDX58 polyclonal antibody in paraffin-embedded Human Rectal Cancer tissue.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cells, using DDX58 polyclonal antibody)

Related Product Information for anti-DDX58 antibody

Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses. dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN. RIG-I does this by using its helicase domain to bind to viral dsRNA, thus transmitting the activation signal for IFN through IkB kinase-related kinases and inducing IFN expression.

NCBI and Uniprot Product Information

• NCBI GI #: 27881482
• NCBI GeneID: 23586
• NCBI Accession #: NP_055129.2
• NCBI GenBank Nucleotide #: NP_055129.2
• UniProt Accession #: O95786
• Molecular Weight: ~ 107kDa
• NCBI Official Full Name: antiviral innate immune response receptor RIG-I
• NCBI Official Synonym Full Names: DExD/H-box helicase 58
• NCBI Official Symbol: DDX58
• NCBI Official Synonym Symbols: RIG1; RIGI; RIG-I; RLR-1; SGMRT2
• NCBI Protein Information: antiviral innate immune response receptor RIG-I
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX58
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX58
• UniProt Synonym Gene Names: RLR-1; RIG-1; RIG-I
• UniProt Entry Name: DDX58_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008]