Western Blot (WB)
(Western Blot (WB) analysis of PMEL pAb at 1:2000 dilutionLane1:A375 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (20ug) Lane3:AML-12 whole cell lysate (20ug) Lane4:L02 whole cell lysate (40ug))
Rabbit PMEL Polyclonal Antibody | anti-PMEL antibody
PMEL Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PMEL; Polyclonal Antibody; PMEL Polyclonal Antibody; Melanocyte protein PMEL; ME20-M; ME20M; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; P1; P100; Premelanosome protein; Silver locus protein homolog; M-alpha; 95 kDa melanocyte-specific secreted glycoprotein; P26; Secreted melanoma-associated ME20 antigen; ME20-S; ME20S; M-beta; D12S53E; PMEL17; SILV; anti-PMEL antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PMEL pAb detects endogenous levels of PMEL protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
661
Applicable Applications for anti-PMEL antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:2000
Immunogen
Synthetic peptide, corresponding to Human PMEL.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of PMEL pAb at 1:2000 dilutionLane1:A375 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (20ug) Lane3:AML-12 whole cell lysate (20ug) Lane4:L02 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of PMEL pAb at 1:2000 dilutionLane1:A375 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (20ug) Lane3:AML-12 whole cell lysate (20ug) Lane4:L02 whole cell lysate (40ug))
Related Product Information for anti-PMEL antibody
Melanocyte protein PMEL, Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~ 70kDa
NCBI Official Full Name
Melanocyte protein PMEL
UniProt Protein Name
Melanocyte protein PMEL
Protein Family
UniProt Gene Name
PMEL
UniProt Synonym Gene Names
D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S
UniProt Entry Name
PMEL_HUMAN
Uniprot Description
SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Vesicle
Chromosomal Location of Human Ortholog: 12q13-q14
Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; plasma membrane; melanosome; integral to membrane; extracellular region
Molecular Function: protein binding
Biological Process: melanin biosynthetic process; melanosome organization and biogenesis
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Product Notes
The PMEL pmel (Catalog #AAA3007700) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PMEL Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PMEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:2000. Researchers should empirically determine the suitability of the PMEL pmel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMEL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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