Rabbit CHD4 Polyclonal Antibody | anti-CHD4 antibody

CHD4 Polyclonal Antibody

Cat. Num. AAA3007355

• Gene Names: CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CHD4; Polyclonal Antibody; CHD4 Polyclonal Antibody; Chromodomain-helicase-DNA-binding protein 4; CHD-4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta; anti-CHD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CHD4 polyclonal antibody detects endogenous levels of CHD4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1912

• Applicable Applications for anti-CHD4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 563-608 of Human CHD4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CHD4 polyclonal antibody at 1:500 dillutionLane1:Hela whole cell lysate (40ug) Lane2:NIH-3T3 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug))

Related Product Information for anti-CHD4 antibody

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Chromatin structure alteration may be brought about by the action of ATP-dependent multiprotein complexes. One such complex is the mSin3 corepressor complex, which contains mSin3, the histone deacetylases HDAC1 and HDAC2, the associated proteins SAP 30 and SAP 18, and the autoantigens Mi2-å and Mi2-beta.

NCBI and Uniprot Product Information

• NCBI GI #: 51599156
• NCBI GeneID: 1108
• NCBI Accession #: NP_001264.2
• NCBI GenBank Nucleotide #: NP_001264.2
• UniProt Accession #: Q14839
• Molecular Weight: ~ 260kDa
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 4 isoform 1
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 4
• NCBI Official Symbol: CHD4
• NCBI Official Synonym Symbols: CHD-4; Mi-2b; SIHIWES; Mi2-BETA
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 4
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 4
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: CHD4
• UniProt Synonym Gene Names: CHD-4
• UniProt Entry Name: CHD4_HUMAN

NCBI Description

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

CHD-4: is an ATP-dependent helicase and a central component of the HDAC-containing nucleosome remodeling and histone deacetylase (NuRD) transcriptional complex. Interacts with MTA1/2, HDAC1, and RbAp46 in the NuRD complex. CHD-4 belongs to the SNF2/RAD54 helicase family of proteins. The NuRD complex, whose subunit composition varies by cell type and in response to physiologic signals, plays central roles in many processes including oncogenesis and metastasis, B cell differentiation, invasive growth of breast cancer, and maintaining the balance between the undifferentiated state and the process of differentiation in embryonic stem cells.

Protein type: Helicase; DNA-binding; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: nucleoplasm; centrosome; protein complex; membrane; nuclear chromatin; cytoplasm; NuRD complex; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; nucleosomal DNA binding; DNA binding; zinc ion binding; microtubule binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; ATP-dependent chromatin remodeling; spindle assembly; DNA duplex unwinding