Rabbit anti-Human ADAM19 Polyclonal Antibody | anti-ADAM19 antibody

ADAM19 Polyclonal Antibody

Cat. Num. AAA3007023

• Gene Names: ADAM19; MLTNB; FKSG34; MADDAM
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ADAM19; Polyclonal Antibody; ADAM19 Polyclonal Antibody; Disintegrin and metalloproteinase domain-containing protein 19; ADAM 19; Meltrin-beta; Metalloprotease and disintegrin dendritic antigen marker; MADDAM; MLTNB; FKSG34; anti-ADAM19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADAM19 polyclonal antibody detects endogenous levels of ADAM19 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 488

• Applicable Applications for anti-ADAM19 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to residues in Human ADAM19
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ADAM19 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADAM19 pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ADAM19 antibody

The ADAM (a disintegrin and metalloprotease) protein family, which includes over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases, plays a role in cell-cell and cell-matrix interface related processes, including fertilization, muscle fusion, secretion of TNFalpha (tumor necrosis factor alpha), and modulation of the neurogenic function of Notch and Delta. The ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in a wide range of mammalian tissues and several are abundantly expressed in the male reproductive tract. Expression of ADAM19, also designated meltrin b, is highest in the peripheral nervous system during embryogenesis, but is also apparent in placenta, brain, heart, lung, leukocytes, and SW480 cells. ADAM19 also serves as a dendritic cell marker. Truncation of ADAM19 in its cysteine-rich domain is necessary to exert its proteolytic activity on specific substrates, including a2-macroglobulin.

NCBI and Uniprot Product Information

• NCBI GI #: 38197238
• NCBI GeneID: 8728
• UniProt Accession #: Q9H013
• Molecular Weight: ~ 105kDa
• NCBI Official Full Name: ADAM19 protein, partial
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 19
• NCBI Official Symbol: ADAM19
• NCBI Official Synonym Symbols: MLTNB; FKSG34; MADDAM
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 19
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 19
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM19
• UniProt Synonym Gene Names: MLTNB; ADAM 19; MADDAM
• UniProt Entry Name: ADA19_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has been demonstrated to be an active metalloproteinase, which may be involved in normal physiological processes such as cell migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. It is proposed to play a role in pathological processes, such as cancer, inflammatory diseases, renal diseases, and Alzheimer's disease. [provided by RefSeq, May 2013]

Uniprot Description

ADAM19: Participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell. Also cleaves alpha-2 macroglobulin. May be involved in osteoblast differentiation and/or osteoblast activity in bone. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; SH3 domain binding

Biological Process: heart development; membrane protein ectodomain proteolysis