Testing Data
(Detection of human TLR3. |Lysates were prepared from Hela cells in |non-reducing sample buffer, resolved by |SDS-PAGE (30ug total protein/lane), and |transferred to an Immobilon-P membrane. The |blot was developed with 2ug/ml |and chemiluminescent detection substrate.)
Mouse anti-Human TLR3 Monoclonal Antibody
TLR3 (Toll Like Receptor 3, Toll-like Receptor 3, TLR 3, TLR-3, CD283, CD283 Antigen)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
TLR3; Monoclonal Antibody; TLR3 (Toll Like Receptor 3; Toll-like Receptor 3; TLR 3; TLR-3; CD283; CD283 Antigen); Anti -TLR3 (Toll Like Receptor 3; anti-TLR3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9H184
Specificity
Recognizes human TLR3.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Applicable Applications for anti-TLR3 antibody
Western Blot (WB)
Application Notes
Dilution: Western Blot: 2ug/ml
Immunogen
NS0 cells transfected with human TLR3 extracellular domain aa27-711; O15455.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Detection of human TLR3. |Lysates were prepared from Hela cells in |non-reducing sample buffer, resolved by |SDS-PAGE (30ug total protein/lane), and |transferred to an Immobilon-P membrane. The |blot was developed with 2ug/ml |and chemiluminescent detection substrate.)
Testing Data
(Detection of human TLR3. |Lysates were prepared from Hela cells in |non-reducing sample buffer, resolved by |SDS-PAGE (30ug total protein/lane), and |transferred to an Immobilon-P membrane. The |blot was developed with 2ug/ml |and chemiluminescent detection substrate.)
Related Product Information for anti-TLR3 antibody
Toll-like receptor 3 (TLR3) is a 103kDa (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. It is localized to the endoplasmic reticulum membrane and endosome, and is expressed at high levels in the placenta and pancreas. It is also detected in the dendritic subpopulation of leukocytes. Human TLR3 is synthesized as a precursor with a 23aa signal sequence, a 681aa luminal region, a 21aa transmembrane region, and a 179aa cytoplasmic region. The luminal region contains 23 canonical and two irregular leucine-rich repeats (LRR), 15 N-linked glycosylation sites, and a Toll/IL-1 receptor (TIR) domain (aa758-896). Human TLR3 shares 83% and 80% aa sequence identity with bovine and mouse TLR3, respectively. TLR3 is involved in the innate immune response to microbial agents.
Product Categories/Family for anti-TLR3 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The TLR3 (Catalog #AAA605516) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TLR3 (Toll Like Receptor 3, Toll-like Receptor 3, TLR 3, TLR-3, CD283, CD283 Antigen) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TLR3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Dilution: Western Blot: 2ug/ml. Researchers should empirically determine the suitability of the TLR3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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