Rabbit CFD Polyclonal Antibody | anti-CFD antibody

CFD Polyclonal Antibody

Cat. Num. AAA3006883

• Gene Names: CFD; DF; ADN; PFD; ADIPSIN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CFD; Polyclonal Antibody; CFD Polyclonal Antibody; Complement factor D; Adipsin; C3 convertase activator; Properdin factor D; DF; PFD; anti-CFD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CFD polyclonal antibody detects endogenous levels of CFD protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 253

• Applicable Applications for anti-CFD antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human CFD
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CFD polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-CFD antibody

Adipsin is the mouse homolog of the previously described human complement Factor D, a serine protease, which is now designated human Adipsin. Human Adipsin is highly expressed in and secreted by adipose tissue, and it has also been found in monocytes and macrophages. Rodent Adipsin has only been detected in high levels in adipose tissue. It has been shown that complement factor B, when complexed with activated complement component C3, is cleaved by Adipsin. While low expression of Adipsin has been confirmed in obese mice with hypothalamic defects, this inverse correlation between Adipsin expression and obesity has not been demonstrated in humans.

NCBI and Uniprot Product Information

• NCBI GI #: 42544239
• NCBI GeneID: 1675
• NCBI Accession #: NP_001919.2
• NCBI GenBank Nucleotide #: NP_001919.2
• UniProt Accession #: P00746
• Molecular Weight: ~ 27kDa
• NCBI Official Full Name: complement factor D isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement factor D
• NCBI Official Symbol: CFD
• NCBI Official Synonym Symbols: DF; ADN; PFD; ADIPSIN
• NCBI Protein Information: complement factor D
• UniProt Protein Name: Complement factor D
• Protein Family: Complement factor
• UniProt Gene Name: CFD
• UniProt Synonym Gene Names: DF; PFD
• UniProt Entry Name: CFAD_HUMAN

NCBI Description

This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease. [provided by RefSeq, Nov 2015]

Uniprot Description

CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.

Protein type: Protease; Secreted, signal peptide; EC 3.4.21.46; Secreted

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: platelet activation; platelet degranulation; complement activation, alternative pathway; innate immune response; blood coagulation; proteolysis; complement activation

Disease: Complement Factor D Deficiency