Rabbit NDUFA1 Polyclonal Antibody | anti-NDUFA1 antibody

NDUFA1 Polyclonal Antibody

Cat. Num. AAA3006808

• Gene Names: NDUFA1; MWFE; ZNF183; CI-MWFE; MC1DN12
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NDUFA1; Polyclonal Antibody; NDUFA1 Polyclonal Antibody; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; Complex I-MWFE; CI-MWFE; anti-NDUFA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NDUFA1 polyclonal antibody detects endogenous levels of NDUFA1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 70

• Applicable Applications for anti-NDUFA1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human NDUFA1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NDUFA1 polyclonal antibody at 1:500 dilution Lane1:HELA whole cell lysate Lane2:RAW264. 7 whole cell lysate Lane3:SP20 whole cell lysate)

Related Product Information for anti-NDUFA1 antibody

NDUFA1 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1), also known as ZNF183 or MWFE, is one of about 45 subunits comprising Complex I of the oxidative phosphorylation electron transport chain. Consisting of 70 amino acids and expressed predominantly in skeletal muscle and heart, NDUFA1 functions as an accessory subunit of the multi-protein mitochondrial membrane respiratory chain NADH dehydrogenase complex (known as Complex I). Complex I plays an important role in the transfer of electrons from NADH to the respiratory chain, a process that is essential for cellular respiration. As a single-pass membrane protein, NDUFA1 localizes to the matrix side of the mitochondrial inner membrane and is a member of the complex I NDUFA1 subunit family. The gene encoding NDUFA1 maps to human chromosome Xq24 and mouse chromosome X A2. NDUFA1 defects may cause mitochondrial Complex I deficiency, a mitochondrial disorder with wide symptoms ranging from cardiomyopathy, myopathy, liver disease and neurological disorders.

NCBI and Uniprot Product Information

• NCBI GI #: 3334271
• NCBI GeneID: 4694
• UniProt Accession #: O15239
• Molecular Weight: ~ 8kDa
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase subunit A1
• NCBI Official Symbol: NDUFA1
• NCBI Official Synonym Symbols: MWFE; ZNF183; CI-MWFE; MC1DN12
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
• UniProt Gene Name: NDUFA1
• UniProt Synonym Gene Names: CI-MWFE
• UniProt Entry Name: NDUA1_HUMAN

NCBI Description

The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008]

Uniprot Description

NDUFA1: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I NDUFA1 subunit family.

Protein type: Oxidoreductase; Mitochondrial; EC 1.6.5.3; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; EC 1.6.99.3

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial membrane; integral to membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone

Disease: Mitochondrial Complex I Deficiency