Rabbit POLR3K Polyclonal Antibody | anti-POLR3K antibody

POLR3K Polyclonal Antibody

Cat. Num. AAA3006414

• Gene Names: POLR3K; C11; My010; RPC10; RPC11; RPC12.5; C11-RNP3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POLR3K; Polyclonal Antibody; POLR3K Polyclonal Antibody; DNA-directed RNA polymerase III subunit RPC10; RNA polymerase III subunit C10; DNA-directed RNA polymerase III subunit K RNA polymerase III 12.5 kDa subunit; RPC12.5; RNA polymerase III subunit C11; HsC11p; RPC11; hRPC11; My010; anti-POLR3K antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POLR3K polyclonal antibody detects endogenous levels of POLR3K protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 108

• Applicable Applications for anti-POLR3K antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human POLR3K.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of POLR3K polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:Mouse lung tissue lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-POLR3K antibody

Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. POLR3K, also known as DNA-directed RNA polymerase III subunit RPC11, is a 108 amino acid nuclear protein that is one of the seventeen subunits that comprise Pol III. Specifically, POLR3K is a DNA-dependent RNA polymerase that uses the four ribonucleoside triphosphates to catalyze the transcription of DNA to RNA. The gene encoding POLR3K maps to human chromosome 16, which encodes over 900 genes in approximately 90 million base pairs and is associated with a variety of genetic disorders.

NCBI and Uniprot Product Information

• NCBI GI #: 597709807
• NCBI GeneID: 51728
• NCBI Accession #: NP_057394.3
• NCBI GenBank Nucleotide #: NP_057394.3
• UniProt Accession #: Q9Y2Y1
• Molecular Weight: ~ 12kDa
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC10
• NCBI Official Synonym Full Names: RNA polymerase III subunit K
• NCBI Official Symbol: POLR3K
• NCBI Official Synonym Symbols: C11; My010; RPC10; RPC11; RPC12.5; C11-RNP3
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC10
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC10
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR3K
• UniProt Synonym Gene Names: RPC11; RNA polymerase III subunit C10; RPC12.5; HsC11p; RPC11; hRPC11
• UniProt Entry Name: RPC10_HUMAN

NCBI Description

This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010]

Uniprot Description

POLR3K: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.

Protein type: DNA-binding; Transcription initiation complex; Transferase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol

Molecular Function: DNA binding; zinc ion binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; positive regulation of interferon type I production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter