Rabbit CYP2A7 Polyclonal Antibody | anti-CYP2A7 antibody

CYP2A7 Polyclonal Antibody

Cat. Num. AAA3005791

• Gene Names: CYP2A7; CPA7; CPAD; CYP2A; CYPIIA7; P450-IIA4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CYP2A7; Polyclonal Antibody; CYP2A7 Polyclonal Antibody; Cytochrome P450 2A7; CYPIIA7; Cytochrome P450 IIA4; anti-CYP2A7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CYP2A7 polyclonal antibody detects endogenous levels of CYP2A7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 494

• Applicable Applications for anti-CYP2A7 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 435-480 of Human CYP2A7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CYP2A7 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:PC12 cell lysate)

Related Product Information for anti-CYP2A7 antibody

The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze reactions involved in both drug metabolism and in the synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies, such as CYP1A and CYP2A, based on their sequence similarities. CYP2A7 (cytochrome P450 2A7), also known as CPA7, CPAD, CYPIIA7 or P450-IIA4, is a 494 amino acid peripheral membrane protein that belongs to the CYP2A subfamily of cytochrome P450 proteins. Localized to the endoplasmic reticulum, CYP2A7 can be induced to high levels in liver in response to foreign compounds, such as carcinogens, pesticides and drugs. CYP2A7 functions as a hydroxylase that uses a heme group to catalyze the oxidation of target flavoproteins.

NCBI and Uniprot Product Information

• NCBI GI #: 15147330
• NCBI GeneID: 1549
• NCBI Accession #: NP_000755.2
• NCBI GenBank Nucleotide #: NP_000755.2
• UniProt Accession #: P20853
• Molecular Weight: ~ 56kDa
• NCBI Official Full Name: cytochrome P450 2A7 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450 family 2 subfamily A member 7
• NCBI Official Symbol: CYP2A7
• NCBI Official Synonym Symbols: CPA7; CPAD; CYP2A; CYPIIA7; P450-IIA4
• NCBI Protein Information: cytochrome P450 2A7
• UniProt Protein Name: Cytochrome P450 2A7
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2A7
• UniProt Entry Name: CP2A7_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum; its substrate has not yet been determined. This gene, which produces two transcript variants, is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2A7: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.14.14.1; Endoplasmic reticulum; Secondary Metabolites Metabolism - caffeine

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding

Biological Process: coumarin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; epoxygenase P450 pathway