Rabbit CPN2 Polyclonal Antibody | anti-CPN2 antibody

CPN2 Polyclonal Antibody

Cat. Num. AAA3005780

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CPN2; Polyclonal Antibody; CPN2 Polyclonal Antibody; Carboxypeptidase N subunit 2; Carboxypeptidase N 83 kDa chain; Carboxypeptidase N large subunit; Carboxypeptidase N polypeptide 2; Carboxypeptidase N regulatory subunit; ACBP; CPN reg; CPN-reg; CPNreg; anti-CPN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CPN2 polyclonal antibody detects endogenous levels of Carboxypeptidase N regulatory subunit protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 545

• Applicable Applications for anti-CPN2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to the 490-535 of Human CPN2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CPN2 polyclonal antibody at 1:500 dilutionLane1:MCF-7 cell lysateLane2:Hela cell lysateLane3:Raw264. 7 cell lysate)

Related Product Information for anti-CPN2 antibody

Carboxypeptidase N (arginine carboxypeptidase or CPN) cleaves basic amino acid residues from the carboxy terminal of peptides and proteins. The enzyme plays a central role in regulating the biologic activity of peptides such as kinins and anaphylatoxins, and therefore is also known as kininase-1 and anaphylatoxin inactivator. CPN is a tetrameric complex consisting of two identical regulatory subunits (CPN reg) and two identical catalytic subunits (CPN cat). The two glycosylated CPN reg subunits protect the two CPN cat subunits and keep them in the circulation. CPN reg is a member of the leucinerich repeat family of proteins and the gene which encodes CPN reg maps to human chromosome 8p22-p23. CPN cat is a member of the regulatory B-type carboxypeptidase group and the gene which encodes CPN cat maps to human chromosome 10.

NCBI and Uniprot Product Information

• NCBI GI #: 256217721
• NCBI Accession #: NP_001073982.2
• NCBI GenBank Nucleotide #: NP_001073982.2
• UniProt Accession #: P22792
• Molecular Weight: ~ 61, 83kDa
• NCBI Official Full Name: carboxypeptidase N subunit 2
• UniProt Protein Name: Carboxypeptidase N subunit 2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPN2
• UniProt Synonym Gene Names: ACBP
• UniProt Entry Name: CPN2_HUMAN

Uniprot Description

CPN2: The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extracellular region

Molecular Function: enzyme regulator activity

Biological Process: protein stabilization; regulation of catalytic activity