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Western Blot (WB) (Western Blot (WB) analysis of CD168 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:NIH-3T3 cell lysateLane3:H9C2 cell lysate)

Rabbit CD168 Polyclonal Antibody | anti-CD168 antibody

CD168 Polyclonal Antibody

Gene Names
HMMR; CD168; IHABP; RHAMM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CD168; Polyclonal Antibody; CD168 Polyclonal Antibody; Hyaluronan mediated motility receptor; Intracellular hyaluronic acid-binding protein; Receptor for hyaluronan-mediated motility; HMMR; IHABP; RHAMM; anti-CD168 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CD168 polyclonal antibody detects endogenous levels of CD168 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
725
Applicable Applications for anti-CD168 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 384-429 of Human CD168.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CD168 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:NIH-3T3 cell lysateLane3:H9C2 cell lysate)

Western Blot (WB) (Western Blot (WB) analysis of CD168 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:NIH-3T3 cell lysateLane3:H9C2 cell lysate)
Related Product Information for anti-CD168 antibody
Hyaluronic acid (HA) is a nonsulfated glycosaminoglycan that regulates cell adhesion and migration. HA effects are mediated through two receptors, CD44 (also designated HCAM) and the receptor of hyaluronic acid mediated motility (RHAMM). RHAMM, also designated intracellular hyaluronic acid binding protein (IHABP) and CD168, is a matrix receptor, which is linked to the plasma membrane by a GPI anchor and regulates cell motility. RHAMM expression is upregulated in malignant lymphoid tissues and is subsequently implicated in tumor progression and metastasis formation, as well as signal transduction. Although still unclear, RHAMM is thought to exist as several isoforms ranging in size. A variant isoform, designated v4, is a protein that when over-expressed, is thought to be the cause of transformation and metastasis formation in fibroblasts.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 85-90kDa
NCBI Official Full Name
hyaluronan mediated motility receptor isoform a
NCBI Official Synonym Full Names
hyaluronan mediated motility receptor
NCBI Official Symbol
HMMR
NCBI Official Synonym Symbols
CD168; IHABP; RHAMM
NCBI Protein Information
hyaluronan mediated motility receptor
UniProt Protein Name
Hyaluronan mediated motility receptor
UniProt Gene Name
HMMR
UniProt Synonym Gene Names
IHABP; RHAMM
UniProt Entry Name
HMMR_HUMAN

NCBI Description

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

HMMR: Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular- regulated kinase (ERK) activity. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: hyaluronic acid binding

Biological Process: glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; hyaluronan metabolic process; hyaluronan catabolic process

Disease: Breast Cancer

Research Articles on CD168

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Product Notes

The CD168 hmmr (Catalog #AAA3005743) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD168 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD168 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CD168 hmmr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD168, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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