Rabbit PKC zeta Polyclonal Antibody | anti-PKC antibody

PKC zeta (Phospho-T410) Polyclonal Antibody

Cat. Num. AAA3004756

• Gene Names: PRKCZ; PKC2; PKC-ZETA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PKC zeta; Polyclonal Antibody; PKC zeta (Phospho-T410) Polyclonal Antibody; Protein kinase C zeta type; nPKC-zeta; PRKCZ; PKC2; anti-PKC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-PKC zeta (T410) polyclonal antibody detects endogenous levels of PKC zeta protein only when phosphorylated at Thr410.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 409

• Applicable Applications for anti-PKC antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human PKC zeta around the phosphorylation site of Threonine 410.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-PKC zeta (T410) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:MCF-7 treated with insulin (100nM/ml,15 minutes) whole cell lysateLane3:The Kidney tissue lysate of RatLane4:The Lung tissue lysate of Mouse)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-PKC zeta (T410) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-PKC antibody

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaii and gamma) and novel (n) PKC isoforms (delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca++. On the other hand, nPKC delta and epsilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities

NCBI and Uniprot Product Information

• NCBI GI #: 75709226
• NCBI GeneID: 5590
• NCBI Accession #: NP_001028753.1
• NCBI GenBank Nucleotide #: NP_001028753.1
• UniProt Accession #: Q05513
• Molecular Weight: ~ 70 to 85kDa
• NCBI Official Full Name: protein kinase C zeta type isoform 2
• NCBI Official Synonym Full Names: protein kinase C zeta
• NCBI Official Symbol: PRKCZ
• NCBI Official Synonym Symbols: PKC2; PKC-ZETA
• NCBI Protein Information: protein kinase C zeta type
• UniProt Protein Name: Protein kinase C zeta type
• UniProt Gene Name: PRKCZ
• UniProt Synonym Gene Names: PKC2
• UniProt Entry Name: KPCZ_HUMAN

NCBI Description

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCZ: an AGC kinase of the PKC family. An atypical PKC: its activity is not regulated by Ca2+, PS, DAG or phorbol esters. Constitutively active.

Protein type: EC 2.7.11.13; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; PKC family; Iota subfamily

Chromosomal Location of Human Ortholog: 1p36.33-p36.2

Cellular Component: tight junction; nuclear matrix; apical cortex; leading edge; nuclear envelope; intercellular junction; cytosol; lipid raft; axon hillock; filamentous actin; membrane; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; microtubule organizing center; cell junction; endosome

Molecular Function: protein serine/threonine kinase activity; protein domain specific binding; protein binding; protein kinase C activity; potassium channel regulator activity; phospholipase binding; insulin receptor substrate binding; metal ion binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: protein heterooligomerization; membrane hyperpolarization; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of interleukin-4 production; positive regulation of glucose import; positive regulation of T-helper 2 cell differentiation; negative regulation of hydrolase activity; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; vesicle transport along microtubule; inflammatory response; positive regulation of cell-matrix adhesion; platelet activation; negative regulation of peptidyl-tyrosine phosphorylation; phospholipase D activation; cell migration; activation of protein kinase B; microtubule cytoskeleton organization and biogenesis; peptidyl-serine phosphorylation; long-term memory; establishment of cell polarity; actin cytoskeleton reorganization; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; blood coagulation; vascular endothelial growth factor receptor signaling pathway; negative regulation of protein complex assembly; negative regulation of apoptosis