Rabbit DD2 Polyclonal Antibody | anti-DD2 antibody

DD2 (Q60) Polyclonal Antibody

Cat. Num. AAA3004316

• Gene Names: AKR1C2; DD; DD2; TDD; BABP; DD-2; DDH2; HBAB; HAKRD; MCDR2; SRXY8; DD/BABP; AKR1C-pseudo
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DD2; Polyclonal Antibody; DD2 (Q60) Polyclonal Antibody; Aldo-keto reductase family 1 member C2; 3-alpha-HSD3; 3alpha-HSD3; HSD3-3alpha; HSD3-alpha3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Trans-1; 2-dihydrobenzene-1; 2-diol dehydrogenase; Type III 3-alpha-hydroxysteroid dehydrogenase; AKR1C2; DDH2; anti-DD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DD2 (Q60) polyclonal antibody detects endogenous levels of DD2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 139

• Applicable Applications for anti-DD2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human DD2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DD2 (Q60) pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug) Lane6:H1792 whole cell lysate (40ug) Lane7:HepG2 whole cell lysate (40ug))

Related Product Information for anti-DD2 antibody

DD1 is also designated AKR1C1, DDH or DDH1, while DD2 also can be designated AKR1C2, dDD, BABP or DDH2. AKR1C3 and 3alpha-HSD are alternate designations for human DD3 (which is referred to as AKR1C18 in rodents), while DD4 also can be called AKR1C4, CD, CHDR or AKR1C6 (in rodents). DD1 and DD2 are 20alpha-HSDs, whereas DD3 and DD4 are the 3alpha- HSDs. The multiple human cytosolic dihydrodiol dehydrogenases are involved in the metabolism of xenobiotics, such as polycyclic aromatic hydrocarbons, pesticides and steroid hormones, and are responsible for the reduction of ketone-containing drugs by using NADH or NADPH as a cofactor. The 20alpha- HSD catalyzes the reaction of Progesterone to the inactive form 20alpha-hydroxyprogesterone.

NCBI and Uniprot Product Information

• NCBI GI #: 207028673
• NCBI GeneID: 1646
• NCBI Accession #: NP_001128713.1
• NCBI GenBank Nucleotide #: NP_001128713.1
• UniProt Accession #: P52895
• Molecular Weight: ~ 37kDa
• NCBI Official Full Name: aldo-keto reductase family 1 member C2 isoform 2
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member C2
• NCBI Official Symbol: AKR1C2
• NCBI Official Synonym Symbols: DD; DD2; TDD; BABP; DD-2; DDH2; HBAB; HAKRD; MCDR2; SRXY8; DD/BABP; AKR1C-pseudo
• NCBI Protein Information: aldo-keto reductase family 1 member C2
• UniProt Protein Name: Aldo-keto reductase family 1 member C2
• UniProt Gene Name: AKR1C2
• UniProt Synonym Gene Names: DDH2; DD-2; DD2; DD/BABP
• UniProt Entry Name: AK1C2_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.357; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.3.1.20

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; carboxylic acid binding; bile acid binding; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity

Biological Process: steroid metabolic process; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; epithelial cell differentiation; digestion; positive regulation of cell proliferation; progesterone metabolic process; prostaglandin metabolic process

Disease: 46,xy Sex Reversal 8