Rabbit AQP0 Polyclonal Antibody | anti-AQP0 antibody

AQP0 (C144) Polyclonal Antibody

Cat. Num. AAA3003847

• Gene Names: MIP; AQP0; LIM1; MP26; MIP26; CTRCT15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AQP0; Polyclonal Antibody; AQP0 (C144) Polyclonal Antibody; Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26; MIP; Aquaporin 0; Aquaporin0; anti-AQP0 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AQP0 (C144) polyclonal antibody detects endogenous levels of AQP0 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 263

• Applicable Applications for anti-AQP0 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 110-160 of Human AQP0.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of AQP0 (C144) pAb at 1:500 dilutionLane1:THP-1 whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of AQP0 (C144) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-AQP0 antibody

AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.

NCBI and Uniprot Product Information

• NCBI GI #: 266537
• NCBI GeneID: 4284
• UniProt Accession #: P30301
• Molecular Weight: ~ 28kDa
• NCBI Official Full Name: Lens fiber major intrinsic protein
• NCBI Official Synonym Full Names: major intrinsic protein of lens fiber
• NCBI Official Symbol: MIP
• NCBI Official Synonym Symbols: AQP0; LIM1; MP26; MIP26; CTRCT15
• NCBI Protein Information: lens fiber major intrinsic protein
• UniProt Protein Name: Lens fiber major intrinsic protein
• UniProt Gene Name: MIP
• UniProt Synonym Gene Names: AQP0; MP26
• UniProt Entry Name: MIP_HUMAN

NCBI Description

Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP0: major intrinsic protein of lens fiber. A member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. May be responsible for regulating the osmolarity of the lens. Defects in MIP are a cause of autosomal recessive congenital cataract.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: intracellular canaliculus; intracellular membrane-bound organelle; integral to plasma membrane; apical plasma membrane; plasma membrane; gap junction

Molecular Function: calmodulin binding; transporter activity; water channel activity; structural constituent of eye lens; glycerol channel activity

Biological Process: lens development in camera-type eye; glycerol transport; cellular water homeostasis; visual perception; canalicular bile acid transport; water transport; response to stimulus; transmembrane transport

Disease: Cataract 15, Multiple Types