Typical Testing Data/Standard Curve (for reference only)
Human UGT2B4 ELISA Kit | UGT2B4 elisa kit
Human UGT2B4 (Uridine Dipho-sphate Glucuronosyltransferase 2 Family Polypeptide B4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UGT2B4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for UGT2B4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UGT2B4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of UGT2B4. You can calculate the concentration of UGT2B4 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
UGT2B4: UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4- nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid. Belongs to the UDP-glycosyltransferase family.
Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - ascorbate and aldarate; EC 2.4.1.17; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Xenobiotic Metabolism - metabolism by cytochrome P450; Membrane protein, integral; Transferase; Lipid Metabolism - androgen and estrogen; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - starch and sucrose
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane
Molecular Function: retinoic acid binding; glucuronosyltransferase activity
Biological Process: flavonoid biosynthetic process; metabolic process; xenobiotic metabolic process; estrogen catabolic process; cellular response to hormone stimulus
Research Articles on UGT2B4
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Product Notes
The Human UGT2B4 ugt2b4 (Catalog #AAA2513671) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2513671 ELISA Kit recognizes Human UGT2B4. It is sometimes possible for the material contained within the vial of "UGT2B4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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