Rabbit ARA70 Polyclonal Antibody | anti-ARA70 antibody

ARA70 (L379) Polyclonal Antibody

Cat. Num. AAA3003256

• Gene Names: NCOA4; RFG; ELE1; PTC3; ARA70
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ARA70; Polyclonal Antibody; ARA70 (L379) Polyclonal Antibody; Nuclear receptor coactivator 4; NCoA-4; Androgen receptor coactivator 70 kDa protein; 70 kDa AR-activator; 70 kDa androgen receptor coactivator; Androgen receptor-associated protein of 70 kDa; Ret-activating protein ELE1; NCOA4; ELE1; RFG; anti-ARA70 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARA70 (L379) polyclonal antibody detects endogenous levels of ARA70 protein
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 650

• Applicable Applications for anti-ARA70 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 348-398 of Human ARA70.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ARA70 (L379) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-ARA70 antibody

-Androgen receptor (AR) coactivator ARA70, also designated NCOA4, RFG and ELE1, is a putative co-activator that specifically enhances the activity of the androgen receptor. In human thyroid carcinomas, the Ret proto-oncogene fuses to ARA70 to form Ret/PTC3 by an intrachromosomal inversion of chromosome 10 in vivo. ARA70 is expressed as two isoforms, ARA70a and ARA70b. The shorter variant, ARA70b, results from an internal 985-bp deletion. ARA70a is widely expressed, and its expression is highest in testis and adipose tissues, whereas ARA70b is solely expressed in the testis. ARA70a can function as a ligand-enhanced co-activator of PPARg in adipocytes. ARA70 may be involved in prostate carcinogenesis and ovarian cancer and may serve as a key mediator of estrogen-androgen synergism.

NCBI and Uniprot Product Information

• NCBI GI #: 223890278
• NCBI GeneID: 8031
• NCBI Accession #: NP_001138732.1
• NCBI GenBank Nucleotide #: NP_001138732.1
• UniProt Accession #: Q13772
• Molecular Weight: ~ 70kDa
• NCBI Official Full Name: nuclear receptor coactivator 4 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor coactivator 4
• NCBI Official Symbol: NCOA4
• NCBI Official Synonym Symbols: RFG; ELE1; PTC3; ARA70
• NCBI Protein Information: nuclear receptor coactivator 4
• UniProt Protein Name: Nuclear receptor coactivator 4
• UniProt Gene Name: NCOA4
• UniProt Synonym Gene Names: ARA70; ELE1; RFG; NCoA-4; 70 kDa AR-activator
• UniProt Entry Name: NCOA4_HUMAN

NCBI Description

This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009]

Uniprot Description

NCOA4: Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. Defects in NCOA4 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Oncoprotein

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: nucleus

Molecular Function: androgen receptor binding; transcription coactivator activity

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; male gonad development; response to hormone stimulus

Disease: Thyroid Carcinoma, Papillary