Rabbit ZBP-89 Polyclonal Antibody | anti-ZBP-89 antibody

ZBP-89 (E84) Polyclonal Antibody

Cat. Num. AAA3002709

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ZBP-89; Polyclonal Antibody; ZBP-89 (E84) Polyclonal Antibody; Zinc finger protein 148; Transcription factor ZBP-89; Zinc finger DNA-binding protein 89; ZNF148; ZBP89; anti-ZBP-89 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ZBP-89 (E84) polyclonal antibody detects endogenous levels of ZBP-89 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 794

• Applicable Applications for anti-ZBP-89 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 53-98 of Human ZBP-89.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ZBP-89 (E84) at 1:500 dilutionLane1:H9C2 whole cell lysate (40ug) Lane2:SP2/0 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:U-87MG whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ZBP-89 (E84) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of ZBP-89 (E84) polyclonal antibody at 1:500 dilutionLane1:COS-7 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:BV2 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Related Product Information for anti-ZBP-89 antibody

ZBP-89, also known as BFCOL1, BERF1 and ZNF 148, is a zinc finger transcription factor that is universally expressed. ZBP-89, a Kruppel-like repressor protein, is the silencer element binding factor for Vimentin. ZBP-89 has been shown to bind to GC-rich DNA elements in promoters for gastrin, ornithine decarboxylase and the cyclin-dependent kinase inhibitor p21 (also designated Cip1 or WAF1). ZBP-89 expression is induced by trans-retinoic acid or butyrate, which also induces terminal differentiation of colon cancer cells. ZBP-89 cooperates with histone acetyltransferase coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor whose associated gene is a target gene of p53. ZBP-89 also regulates cell proliferation, in part, through its ability to directly bind the p53 protein and retard its nuclear export. Elevated levels of ZBP-89 induce growth arrest and apoptosis in human gastrointestinal cells.

NCBI and Uniprot Product Information

• NCBI GI #: 145386566
• NCBI Accession #: NP_068799.2
• NCBI GenBank Nucleotide #: NP_068799.2
• UniProt Accession #: Q9UQR1
• Molecular Weight: ~ 89kDa
• NCBI Official Full Name: zinc finger protein 148 isoform a
• UniProt Protein Name: Zinc finger protein 148
• UniProt Gene Name: ZNF148
• UniProt Synonym Gene Names: ZBP89
• UniProt Entry Name: ZN148_HUMAN

Uniprot Description

ZNF148: a transcription factor. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: nucleoplasm; Golgi apparatus

Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; substantia nigra development; gamete generation; cellular defense response; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent