Rabbit PKA Ibeta reg Polyclonal Antibody | anti-PKA antibody

PKA Ibeta reg (F138) Polyclonal Antibody

Cat. Num. AAA3002385

• Gene Names: PRKAR1B; PRKAR1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PKA Ibeta reg; Polyclonal Antibody; PKA Ibeta reg (F138) Polyclonal Antibody; cAMP-dependent protein kinase type I-beta regulatory subunit; PRKAR1B; anti-PKA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PKA Ibeta reg (F138) polyclonal antibody detects endogenous levels of PKA Ibeta reg protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 381

• Applicable Applications for anti-PKA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 120-160 of Human PKA Ibeta reg.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PKA Ibeta reg (F138) pAb at 1:500 dilutionLane1:The Brain tissue lysate of Mouse (10ug) Lane2:The Brain tissue lysate of Rat (20ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:K562 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PKA Ibeta reg (F138) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of KAP1 pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug))

Related Product Information for anti-PKA antibody

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Calpha, Cbeta and Cgamma, that each represent specific gene products. Calpha and Cbeta are closely related (93% amino acid sequence similarity), whereas Cgamma displays 83% and 79% similarity to Calpha and Cbeta, respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 258613969
• NCBI GeneID: 5575
• NCBI Accession #: NP_001158230.1
• NCBI GenBank Nucleotide #: NP_001158230.1
• UniProt Accession #: P31321
• Molecular Weight: ~ 43kDa
• NCBI Official Full Name: cAMP-dependent protein kinase type I-beta regulatory subunit
• NCBI Official Synonym Full Names: protein kinase cAMP-dependent type I regulatory subunit beta
• NCBI Official Symbol: PRKAR1B
• NCBI Official Synonym Symbols: PRKAR1
• NCBI Protein Information: cAMP-dependent protein kinase type I-beta regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type I-beta regulatory subunit
• Protein Family: Protein lysine acetyltransferase
• UniProt Gene Name: PRKAR1B
• UniProt Entry Name: KAP1_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

PKAR1B: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Belongs to the cAMP-dependent kinase regulatory chain family.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: plasma membrane; cytosol; cAMP-dependent protein kinase complex

Molecular Function: cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; protein amino acid phosphorylation; learning and/or memory; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; blood coagulation; regulation of insulin secretion; transmembrane transport