Rabbit JIP-2 Polyclonal Antibody | anti-JIP-2 antibody

JIP-2 (N601) Polyclonal Antibody

Cat. Num. AAA3002103

• Gene Names: MAPK8IP2; IB2; IB-2; JIP2; PRKM8IPL
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: JIP-2; Polyclonal Antibody; JIP-2 (N601) Polyclonal Antibody; C-Jun-amino-terminal kinase-interacting protein 2; JNK-interacting protein 2; Islet-brain-2; IB-2; JNK MAP kinase scaffold protein 2; Mitogen-activated protein kinase 8-interacting protein 2; MAPK8IP2; IB2; JIP2; PRKM8IPL; anti-JIP-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: JIP-2 (N601) polyclonal antibody detects endogenous levels of JIP-2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 824

• Applicable Applications for anti-JIP-2 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 572-622 of Human JIP-2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of JIP-2 (N601) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-JIP-2 antibody

c-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites, Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members have been identified as JNK2 and JNK3. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.

NCBI and Uniprot Product Information

• NCBI GI #: 6912492
• NCBI GeneID: 23542
• NCBI Accession #: NP_036456.1
• NCBI GenBank Nucleotide #: NP_036456.1
• UniProt Accession #: Q13387
• Molecular Weight: ~ 88kDa
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 2
• NCBI Official Symbol: MAPK8IP2
• NCBI Official Synonym Symbols: IB2; IB-2; JIP2; PRKM8IPL
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 2
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 2
• UniProt Gene Name: MAPK8IP2
• UniProt Synonym Gene Names: IB2; JIP2; PRKM8IPL; JIP-2; JNK-interacting protein 2; IB-2
• UniProt Entry Name: JIP2_HUMAN

NCBI Description

This gene encodes a scaffold protein that is thought to be involved in the regulation of the c-Jun amino-terminal kinase signaling pathway. This protein has been shown to interact with and regulate the activity of MAPK8/JNK1 and MAP2K7/MKK7 kinases. [provided by RefSeq, Jun 2017]

Uniprot Description

MAPK8IP2: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Belongs to the JIP scaffold family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit; Activator

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: protein complex; cell soma; cytoplasm

Molecular Function: MAP-kinase scaffold activity; protein binding; kinesin binding; beta-amyloid binding; protein complex binding; structural molecule activity; protein kinase binding; protein kinase activator activity

Biological Process: nonassociative learning; regulation of synaptic transmission, glutamatergic; behavioral fear response; dendrite morphogenesis; MAPKKK cascade; mating behavior; signal complex assembly; social behavior; regulation of JNK cascade; positive regulation of stress-activated MAPK cascade; positive regulation of protein kinase activity; JNK cascade; regulation of receptor activity; regulation of excitatory postsynaptic membrane potential